miRNA: 3'- gUCGG----AAGCGUG-CGCC---UCCGCCGCGu -5'
miRNA: 3'- -GUCGGaag--CGUGCgCCUCCGCCgCGU- -5'
miRNA: 3'- -GUCGGa--AGCGUGCGcCUCCGCcGCGU- -5'
miRNA: 3'- -GUCGGAAGCGUGCGCCUCCGCCGCGU- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13438 | 3' | -62.2 | NC_003409.1 | + | 87909 | 0.73 | 0.246086 |
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Target: 5'- aAGCCguguUUCGUGCuGCGGcuuGGGCGGCGCc -3' miRNA: 3'- gUCGG----AAGCGUG-CGCC---UCCGCCGCGu -5' |
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| 13438 | 3' | -62.2 | NC_003409.1 | + | 131249 | 0.73 | 0.24038 |
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Target: 5'- gCGGCCacacgGCGCGgGGAGGUGGgGCAc -3' miRNA: 3'- -GUCGGaag--CGUGCgCCUCCGCCgCGU- -5' |
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| 13438 | 3' | -62.2 | NC_003409.1 | + | 38608 | 0.74 | 0.20348 |
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Target: 5'- cCAGCCaagUUGCGCGCcGAGGCGcCGCAg -3' miRNA: 3'- -GUCGGa--AGCGUGCGcCUCCGCcGCGU- -5' |
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| 13438 | 3' | -62.2 | NC_003409.1 | + | 109449 | 1.07 | 0.000841 |
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Target: 5'- aCAGCCUUCGCACGCGGAGGCGGCGCAc -3' miRNA: 3'- -GUCGGAAGCGUGCGCCUCCGCCGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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