miRNA: 3'- caGUGCGACGGU--------------UGU--------------UACgAGUUCCG- -5'
miRNA: 3'- cagUGCGaCGGUugUUaCGa--GUUCCG- -5'
miRNA: 3'- -CAGu--GCgaCGGUUGUUACGaGU--UCCG- -5'
miRNA: 3'- cagUGCGAC-GGUUGuuACG-------AGUUCCG- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13438 | 5' | -51.5 | NC_003409.1 | + | 130518 | 0.66 | 0.975995 |
|
Target: 5'- -aCAUGCUGCCAcugccacggauggaACAucagagaugggucuAUGaUCAGGGCa -3' miRNA: 3'- caGUGCGACGGU--------------UGU--------------UACgAGUUCCG- -5' |
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| 13438 | 5' | -51.5 | NC_003409.1 | + | 131201 | 0.66 | 0.982128 |
|
Target: 5'- --aACGCgGCCAugAGcGCguaCGGGGCa -3' miRNA: 3'- cagUGCGaCGGUugUUaCGa--GUUCCG- -5' |
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| 13438 | 5' | -51.5 | NC_003409.1 | + | 131501 | 0.66 | 0.972116 |
|
Target: 5'- uGUCccuCGggGCCAACGGUGCcCAguGGGUg -3' miRNA: 3'- -CAGu--GCgaCGGUUGUUACGaGU--UCCG- -5' |
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| 13438 | 5' | -51.5 | NC_003409.1 | + | 132814 | 0.71 | 0.820327 |
|
Target: 5'- --gGCGCUGuCCGAUggUGUccgcgucUCGGGGCa -3' miRNA: 3'- cagUGCGAC-GGUUGuuACG-------AGUUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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