Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1344 | 3' | -52.3 | NC_001335.1 | + | 42119 | 0.66 | 0.860136 |
Target: 5'- uGUGuccGAUGuGUagAUCGGACgugaACCAGGUg -3' miRNA: 3'- gCACu--CUACuCG--UAGUCUG----UGGUCCG- -5' |
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1344 | 3' | -52.3 | NC_001335.1 | + | 15522 | 0.66 | 0.842745 |
Target: 5'- aCGUcGuGGUGGGCAgCAG-UACCGGGg -3' miRNA: 3'- -GCA-CuCUACUCGUaGUCuGUGGUCCg -5' |
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1344 | 3' | -52.3 | NC_001335.1 | + | 26429 | 0.66 | 0.84185 |
Target: 5'- aGUGGGcUGcGGCAUCGGaggaggcGCgACCGGGUa -3' miRNA: 3'- gCACUCuAC-UCGUAGUC-------UG-UGGUCCG- -5' |
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1344 | 3' | -52.3 | NC_001335.1 | + | 41083 | 0.66 | 0.823482 |
Target: 5'- --cGAGAUGGGcCGUgAGACAggcucaugcgucgUCGGGCu -3' miRNA: 3'- gcaCUCUACUC-GUAgUCUGU-------------GGUCCG- -5' |
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1344 | 3' | -52.3 | NC_001335.1 | + | 11906 | 0.67 | 0.795365 |
Target: 5'- gGUGAGGUcGGCGUUGGA-GCCGGuGCc -3' miRNA: 3'- gCACUCUAcUCGUAGUCUgUGGUC-CG- -5' |
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1344 | 3' | -52.3 | NC_001335.1 | + | 7437 | 0.68 | 0.754167 |
Target: 5'- -aUGGGAcGAGUAcCAGAagguCCAGGCa -3' miRNA: 3'- gcACUCUaCUCGUaGUCUgu--GGUCCG- -5' |
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1344 | 3' | -52.3 | NC_001335.1 | + | 14401 | 0.68 | 0.754167 |
Target: 5'- gGUuGGAcGAGCGUCGGccACACCagccgAGGCa -3' miRNA: 3'- gCAcUCUaCUCGUAGUC--UGUGG-----UCCG- -5' |
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1344 | 3' | -52.3 | NC_001335.1 | + | 42878 | 0.68 | 0.737057 |
Target: 5'- uCGUGccugccuuagagggaAGGUGAGUAUCGGugGCgucaAGGUg -3' miRNA: 3'- -GCAC---------------UCUACUCGUAGUCugUGg---UCCG- -5' |
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1344 | 3' | -52.3 | NC_001335.1 | + | 13105 | 0.68 | 0.732733 |
Target: 5'- gGUGcucuGAUGAGCcUCcucGACACCGGuGCc -3' miRNA: 3'- gCACu---CUACUCGuAGu--CUGUGGUC-CG- -5' |
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1344 | 3' | -52.3 | NC_001335.1 | + | 10275 | 0.68 | 0.721849 |
Target: 5'- uCGUGcAGGgcUGGGCGagGGucgcCACCGGGCg -3' miRNA: 3'- -GCAC-UCU--ACUCGUagUCu---GUGGUCCG- -5' |
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1344 | 3' | -52.3 | NC_001335.1 | + | 20910 | 0.68 | 0.721849 |
Target: 5'- aCG-GAGc--GGCGUCAGACACCuugaGGGUg -3' miRNA: 3'- -GCaCUCuacUCGUAGUCUGUGG----UCCG- -5' |
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1344 | 3' | -52.3 | NC_001335.1 | + | 18345 | 0.69 | 0.707563 |
Target: 5'- gGUGucaauGGUGAGCGUuucaacuugacgacCGGugACCAGGg -3' miRNA: 3'- gCACu----CUACUCGUA--------------GUCugUGGUCCg -5' |
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1344 | 3' | -52.3 | NC_001335.1 | + | 8557 | 0.69 | 0.685336 |
Target: 5'- --aGAGAUGcAGCAacugcuggcucacgUCGGAUACCccAGGCu -3' miRNA: 3'- gcaCUCUAC-UCGU--------------AGUCUGUGG--UCCG- -5' |
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1344 | 3' | -52.3 | NC_001335.1 | + | 20660 | 0.7 | 0.621125 |
Target: 5'- gCGUGcGAUGGGCAUCucgcucccGAUcuCCGGGCu -3' miRNA: 3'- -GCACuCUACUCGUAGu-------CUGu-GGUCCG- -5' |
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1344 | 3' | -52.3 | NC_001335.1 | + | 25983 | 0.72 | 0.510258 |
Target: 5'- cCGUGGcGAcGAGCGUCAGGCcgucgaccACCAagGGCa -3' miRNA: 3'- -GCACU-CUaCUCGUAGUCUG--------UGGU--CCG- -5' |
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1344 | 3' | -52.3 | NC_001335.1 | + | 29656 | 0.73 | 0.437727 |
Target: 5'- --gGAGAUGGGCGUCaAGAuCACCGGucGCa -3' miRNA: 3'- gcaCUCUACUCGUAG-UCU-GUGGUC--CG- -5' |
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1344 | 3' | -52.3 | NC_001335.1 | + | 10396 | 1 | 0.007781 |
Target: 5'- cCGUGAGAUGAGCAU-AGACACCAGGCg -3' miRNA: 3'- -GCACUCUACUCGUAgUCUGUGGUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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