Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1344 | 5' | -64.2 | NC_001335.1 | + | 32516 | 0.66 | 0.31928 |
Target: 5'- aCGCCCGacuUGuaGACCauCUCGCCguGCu -3' miRNA: 3'- cGCGGGCc--ACcgCUGG--GAGCGGguCG- -5' |
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1344 | 5' | -64.2 | NC_001335.1 | + | 50533 | 0.66 | 0.31928 |
Target: 5'- cGUGCCCGacGUGGCcgucaugUCUUCGCCCcagucGGCg -3' miRNA: 3'- -CGCGGGC--CACCGcu-----GGGAGCGGG-----UCG- -5' |
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1344 | 5' | -64.2 | NC_001335.1 | + | 51641 | 0.66 | 0.318544 |
Target: 5'- -gGCCgGGUGGCugucaaGCCCUaaucgcaaauuggUGCCCuAGCu -3' miRNA: 3'- cgCGGgCCACCGc-----UGGGA-------------GCGGG-UCG- -5' |
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1344 | 5' | -64.2 | NC_001335.1 | + | 38843 | 0.66 | 0.311978 |
Target: 5'- -gGCCCGuuUGaaGAacucgaugcaguCCCUCGCCCAGCc -3' miRNA: 3'- cgCGGGCc-ACcgCU------------GGGAGCGGGUCG- -5' |
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1344 | 5' | -64.2 | NC_001335.1 | + | 32690 | 0.66 | 0.311978 |
Target: 5'- aGCGCCuugaacucggCGGUGuuGACCUUgG-CCAGCa -3' miRNA: 3'- -CGCGG----------GCCACcgCUGGGAgCgGGUCG- -5' |
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1344 | 5' | -64.2 | NC_001335.1 | + | 7038 | 0.66 | 0.304803 |
Target: 5'- aGCGCggCGGUGaacgGAUCCUUGCcCCAGCc -3' miRNA: 3'- -CGCGg-GCCACcg--CUGGGAGCG-GGUCG- -5' |
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1344 | 5' | -64.2 | NC_001335.1 | + | 25892 | 0.66 | 0.297756 |
Target: 5'- gGUGCCCuuGGUGGUcgacGGCCUgacgcucgUCGCCaCGGUc -3' miRNA: 3'- -CGCGGG--CCACCG----CUGGG--------AGCGG-GUCG- -5' |
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1344 | 5' | -64.2 | NC_001335.1 | + | 25129 | 0.66 | 0.277374 |
Target: 5'- gGCG-CUGGcGGCuGCCUucuUCGCCCGGUc -3' miRNA: 3'- -CGCgGGCCaCCGcUGGG---AGCGGGUCG- -5' |
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1344 | 5' | -64.2 | NC_001335.1 | + | 17235 | 0.67 | 0.270832 |
Target: 5'- cGCGCCUGcGaccUGGCcgagGGCCgUCG-CCAGCg -3' miRNA: 3'- -CGCGGGC-C---ACCG----CUGGgAGCgGGUCG- -5' |
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1344 | 5' | -64.2 | NC_001335.1 | + | 46942 | 0.67 | 0.270832 |
Target: 5'- -gGCCUGGcgcagcagcuUGGCGA-CCUUGUCCuGCa -3' miRNA: 3'- cgCGGGCC----------ACCGCUgGGAGCGGGuCG- -5' |
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1344 | 5' | -64.2 | NC_001335.1 | + | 46009 | 0.67 | 0.258121 |
Target: 5'- cCGuCCCGGUugucguugucccGGCGGgCUUCGCCCuucGGUg -3' miRNA: 3'- cGC-GGGCCA------------CCGCUgGGAGCGGG---UCG- -5' |
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1344 | 5' | -64.2 | NC_001335.1 | + | 20486 | 0.67 | 0.257498 |
Target: 5'- -gGCCCuggugaacaucGGUGGCGACcugcugaCCUCGUucaucaacagCCAGCu -3' miRNA: 3'- cgCGGG-----------CCACCGCUG-------GGAGCG----------GGUCG- -5' |
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1344 | 5' | -64.2 | NC_001335.1 | + | 50835 | 0.67 | 0.248306 |
Target: 5'- cGCGCCCaaGGUGcGCcGCCCccggccgacgacucgUCGCUgGGCc -3' miRNA: 3'- -CGCGGG--CCAC-CGcUGGG---------------AGCGGgUCG- -5' |
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1344 | 5' | -64.2 | NC_001335.1 | + | 50927 | 0.67 | 0.239974 |
Target: 5'- cUGCCCGGUGacgccCGGCCUgUCGgCCUGGCu -3' miRNA: 3'- cGCGGGCCACc----GCUGGG-AGC-GGGUCG- -5' |
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1344 | 5' | -64.2 | NC_001335.1 | + | 5940 | 0.68 | 0.233591 |
Target: 5'- uCuaCCGGUGGCaGCCCaUCGgcaacuaCCCGGCa -3' miRNA: 3'- cGcgGGCCACCGcUGGG-AGC-------GGGUCG- -5' |
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1344 | 5' | -64.2 | NC_001335.1 | + | 27558 | 0.68 | 0.222904 |
Target: 5'- aGC-CCCGGUGGa---CUUCGCCCAcuGCu -3' miRNA: 3'- -CGcGGGCCACCgcugGGAGCGGGU--CG- -5' |
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1344 | 5' | -64.2 | NC_001335.1 | + | 12137 | 0.68 | 0.222904 |
Target: 5'- cGUGCCCGa-GGCGACgUggUCGCUCAGg -3' miRNA: 3'- -CGCGGGCcaCCGCUGgG--AGCGGGUCg -5' |
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1344 | 5' | -64.2 | NC_001335.1 | + | 43588 | 0.68 | 0.219073 |
Target: 5'- uGUGCCCGuucgaggcucaucucGCGGCCCUCGgCCAGa -3' miRNA: 3'- -CGCGGGCcac------------CGCUGGGAGCgGGUCg -5' |
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1344 | 5' | -64.2 | NC_001335.1 | + | 29264 | 0.69 | 0.201762 |
Target: 5'- uGCGCCUGGUagucgacugaGGCcAUCacaUCGCCUGGCu -3' miRNA: 3'- -CGCGGGCCA----------CCGcUGGg--AGCGGGUCG- -5' |
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1344 | 5' | -64.2 | NC_001335.1 | + | 15377 | 0.69 | 0.177804 |
Target: 5'- -aGUUCGGcggGGCGAUCCUCGCagaccugcuCCAGUa -3' miRNA: 3'- cgCGGGCCa--CCGCUGGGAGCG---------GGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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