Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13443 | 5' | -55.6 | NC_003409.1 | + | 131096 | 0.66 | 0.920958 |
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Target: 5'- cUGGugG-CCCUGgGUguaGUGGGCCGGu -3' miRNA: 3'- cACUugCaGGGGCgCAg--CAUUCGGCU- -5' |
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| 13443 | 5' | -55.6 | NC_003409.1 | + | 49917 | 0.66 | 0.903124 |
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Target: 5'- -cGGACGcCUCCGUGUUGUucguauacgcgGGGUCGAu -3' miRNA: 3'- caCUUGCaGGGGCGCAGCA-----------UUCGGCU- -5' |
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| 13443 | 5' | -55.6 | NC_003409.1 | + | 131416 | 0.66 | 0.896708 |
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Target: 5'- cGUGccccACcUCCCCGCGcCGUGuGGCCGc -3' miRNA: 3'- -CACu---UGcAGGGGCGCaGCAU-UCGGCu -5' |
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| 13443 | 5' | -55.6 | NC_003409.1 | + | 46092 | 0.68 | 0.820623 |
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Target: 5'- -cGAACGUaCCUCGCGcCGUAccaCCGAa -3' miRNA: 3'- caCUUGCA-GGGGCGCaGCAUuc-GGCU- -5' |
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| 13443 | 5' | -55.6 | NC_003409.1 | + | 110083 | 0.71 | 0.687577 |
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Target: 5'- -aGAGCGUUCCUGCGcCu--GGCCGAc -3' miRNA: 3'- caCUUGCAGGGGCGCaGcauUCGGCU- -5' |
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| 13443 | 5' | -55.6 | NC_003409.1 | + | 105837 | 0.72 | 0.61607 |
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Target: 5'- -gGAGCGUCUuuGCGUCcuccguugGUAuGCCGAg -3' miRNA: 3'- caCUUGCAGGggCGCAG--------CAUuCGGCU- -5' |
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| 13443 | 5' | -55.6 | NC_003409.1 | + | 112029 | 1.08 | 0.003538 |
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Target: 5'- gGUGAACGUCCCCGCGUCGUAAGCCGAc -3' miRNA: 3'- -CACUUGCAGGGGCGCAGCAUUCGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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