Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13445 | 5' | -48.6 | NC_003409.1 | + | 87318 | 0.66 | 0.997879 |
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Target: 5'- -cGGCG-CACCACCcaccccAGAGGUgGCAGAg -3' miRNA: 3'- acCCGCaGUGGUGG------UUUUUAaCGUUU- -5' |
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| 13445 | 5' | -48.6 | NC_003409.1 | + | 91735 | 0.68 | 0.987945 |
|
Target: 5'- gGGGUGUCcCCugCAA----UGCAAGg -3' miRNA: 3'- aCCCGCAGuGGugGUUuuuaACGUUU- -5' |
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| 13445 | 5' | -48.6 | NC_003409.1 | + | 95377 | 0.69 | 0.980084 |
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Target: 5'- aGGGgGUCGCCGCCAAcgucccGCu-- -3' miRNA: 3'- aCCCgCAGUGGUGGUUuuuaa-CGuuu -5' |
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| 13445 | 5' | -48.6 | NC_003409.1 | + | 102297 | 0.69 | 0.974393 |
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Target: 5'- cUGGGCagcuacaGUC-CCACCGgcuuggccuucacAGAAUUGCAAAa -3' miRNA: 3'- -ACCCG-------CAGuGGUGGU-------------UUUUAACGUUU- -5' |
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| 13445 | 5' | -48.6 | NC_003409.1 | + | 107962 | 0.66 | 0.996851 |
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Target: 5'- gGGGCGUgC-CCACCGuggccugcUGCAAAu -3' miRNA: 3'- aCCCGCA-GuGGUGGUuuuua---ACGUUU- -5' |
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| 13445 | 5' | -48.6 | NC_003409.1 | + | 110009 | 0.68 | 0.983589 |
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Target: 5'- cGGGCGcCACCGCCucccgccGCGGc -3' miRNA: 3'- aCCCGCaGUGGUGGuuuuuaaCGUUu -5' |
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| 13445 | 5' | -48.6 | NC_003409.1 | + | 113877 | 1.07 | 0.013432 |
|
Target: 5'- uUGGGCGUCACCACCAAAAAUUGCAAAa -3' miRNA: 3'- -ACCCGCAGUGGUGGUUUUUAACGUUU- -5' |
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| 13445 | 5' | -48.6 | NC_003409.1 | + | 119732 | 0.7 | 0.953898 |
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Target: 5'- aUGGGCGcCGCCAUCuuGAAUuUGCu-- -3' miRNA: 3'- -ACCCGCaGUGGUGGuuUUUA-ACGuuu -5' |
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| 13445 | 5' | -48.6 | NC_003409.1 | + | 133308 | 0.67 | 0.99498 |
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Target: 5'- aGGGCGUU---GCCAGGAGUggugGCGAu -3' miRNA: 3'- aCCCGCAGuggUGGUUUUUAa---CGUUu -5' |
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| 13445 | 5' | -48.6 | NC_003409.1 | + | 133546 | 0.67 | 0.99084 |
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Target: 5'- aGGGCGUacaGCagGCCGAGAugcUGCAGGa -3' miRNA: 3'- aCCCGCAg--UGg-UGGUUUUua-ACGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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