Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13446 | 5' | -53.3 | NC_003409.1 | + | 128323 | 0.66 | 0.972399 |
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Target: 5'- cUGUACGUggCGACgUUUACGGGAccgGGGAg -3' miRNA: 3'- cAUAUGCAg-GCUG-AGGUGCCCU---UCCU- -5' |
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| 13446 | 5' | -53.3 | NC_003409.1 | + | 88460 | 0.66 | 0.959461 |
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Target: 5'- -gAUGCGUUgGugUCgGCGGGAAa-- -3' miRNA: 3'- caUAUGCAGgCugAGgUGCCCUUccu -5' |
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| 13446 | 5' | -53.3 | NC_003409.1 | + | 85889 | 0.67 | 0.947369 |
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Target: 5'- aUAUugGUCCaGCggaUCCGCGGuGAuAGGAu -3' miRNA: 3'- cAUAugCAGGcUG---AGGUGCC-CU-UCCU- -5' |
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| 13446 | 5' | -53.3 | NC_003409.1 | + | 109613 | 0.67 | 0.947369 |
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Target: 5'- -cGUGCG-CCGcCUCCGCGuGcGAAGGc -3' miRNA: 3'- caUAUGCaGGCuGAGGUGC-C-CUUCCu -5' |
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| 13446 | 5' | -53.3 | NC_003409.1 | + | 71273 | 0.67 | 0.94286 |
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Target: 5'- -cAUACG-CUGGCuUCCcCGGGggGGc -3' miRNA: 3'- caUAUGCaGGCUG-AGGuGCCCuuCCu -5' |
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| 13446 | 5' | -53.3 | NC_003409.1 | + | 26038 | 0.67 | 0.94286 |
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Target: 5'- ----uUGUCgGAC-CCGCGGGcGGGAc -3' miRNA: 3'- cauauGCAGgCUGaGGUGCCCuUCCU- -5' |
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| 13446 | 5' | -53.3 | NC_003409.1 | + | 34430 | 0.69 | 0.862047 |
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Target: 5'- --uUACGguggCUGGC-CUGCGGGggGGAu -3' miRNA: 3'- cauAUGCa---GGCUGaGGUGCCCuuCCU- -5' |
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| 13446 | 5' | -53.3 | NC_003409.1 | + | 109404 | 0.7 | 0.837898 |
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Target: 5'- -cAUugGaggaaGACUCUGCGGGAAGGGg -3' miRNA: 3'- caUAugCagg--CUGAGGUGCCCUUCCU- -5' |
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| 13446 | 5' | -53.3 | NC_003409.1 | + | 16975 | 0.7 | 0.820818 |
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Target: 5'- uGUGUGUGUCCGGCUCCaauaugACGGGAu--- -3' miRNA: 3'- -CAUAUGCAGGCUGAGG------UGCCCUuccu -5' |
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| 13446 | 5' | -53.3 | NC_003409.1 | + | 114689 | 0.72 | 0.74611 |
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Target: 5'- ---cAUGUUgGGUUCCACGGGGAGGGg -3' miRNA: 3'- cauaUGCAGgCUGAGGUGCCCUUCCU- -5' |
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| 13446 | 5' | -53.3 | NC_003409.1 | + | 114040 | 1.09 | 0.005469 |
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Target: 5'- gGUAUACGUCCGACUCCACGGGAAGGAc -3' miRNA: 3'- -CAUAUGCAGGCUGAGGUGCCCUUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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