Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13447 | 3' | -46.5 | NC_003409.1 | + | 82428 | 0.66 | 0.999916 |
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Target: 5'- -gGGGGUGGGaUUCCcuGGGCcgACUGGg -3' miRNA: 3'- ggCUCUACUC-GAGGuuUUUGa-UGACC- -5' |
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| 13447 | 3' | -46.5 | NC_003409.1 | + | 94182 | 0.69 | 0.998858 |
|
Target: 5'- gCCGGuugucGGGCUgCGGAAACUugUGGu -3' miRNA: 3'- -GGCUcua--CUCGAgGUUUUUGAugACC- -5' |
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| 13447 | 3' | -46.5 | NC_003409.1 | + | 104363 | 0.68 | 0.999245 |
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Target: 5'- cCUGuccGAUGAGC-CCAuAAAACU-CUGGa -3' miRNA: 3'- -GGCu--CUACUCGaGGU-UUUUGAuGACC- -5' |
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| 13447 | 3' | -46.5 | NC_003409.1 | + | 108303 | 0.68 | 0.999069 |
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Target: 5'- aCGAGAcGGGuCUCgAGGGACUGucCUGGc -3' miRNA: 3'- gGCUCUaCUC-GAGgUUUUUGAU--GACC- -5' |
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| 13447 | 3' | -46.5 | NC_003409.1 | + | 112456 | 0.66 | 0.999953 |
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Target: 5'- --aAGGUGAGagaccccgugaUCCAGGAGCgACUGGa -3' miRNA: 3'- ggcUCUACUCg----------AGGUUUUUGaUGACC- -5' |
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| 13447 | 3' | -46.5 | NC_003409.1 | + | 114924 | 1.14 | 0.012224 |
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Target: 5'- aCCGAGAUGAGCUCCAAAAACUACUGGc -3' miRNA: 3'- -GGCUCUACUCGAGGUUUUUGAUGACC- -5' |
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| 13447 | 3' | -46.5 | NC_003409.1 | + | 121241 | 0.67 | 0.999814 |
|
Target: 5'- aCGAGGuuUGAGaggcguagacaUCCGugucGACUGCUGGa -3' miRNA: 3'- gGCUCU--ACUCg----------AGGUuu--UUGAUGACC- -5' |
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| 13447 | 3' | -46.5 | NC_003409.1 | + | 131116 | 0.84 | 0.530545 |
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Target: 5'- gCGAGGUGGGCuUCCA---GCUGCUGGu -3' miRNA: 3'- gGCUCUACUCG-AGGUuuuUGAUGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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