Results 41 - 45 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13448 | 5' | -63.9 | NC_003409.1 | + | 78443 | 0.68 | 0.385053 |
Target: 5'- gGCUACCgGGCUgCGCCagCCAcgGCUGCCc -3' miRNA: 3'- -CGGUGG-CCGGgGUGG--GGUa-CGACGGu -5' |
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13448 | 5' | -63.9 | NC_003409.1 | + | 63266 | 0.68 | 0.385053 |
Target: 5'- gGCCugucCCGuauacCCCCACCCUGUGgUGCCc -3' miRNA: 3'- -CGGu---GGCc----GGGGUGGGGUACgACGGu -5' |
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13448 | 5' | -63.9 | NC_003409.1 | + | 35392 | 0.68 | 0.393115 |
Target: 5'- aCCGCCGGCUCCucaaguACUCCAccCUGCCc -3' miRNA: 3'- cGGUGGCCGGGG------UGGGGUacGACGGu -5' |
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13448 | 5' | -63.9 | NC_003409.1 | + | 35529 | 0.68 | 0.421329 |
Target: 5'- uCCGCCGcGCCCCACCgaguccgcuccaaaaCCGccuuCUGCCAu -3' miRNA: 3'- cGGUGGC-CGGGGUGG---------------GGUac--GACGGU- -5' |
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13448 | 5' | -63.9 | NC_003409.1 | + | 24473 | 0.66 | 0.529674 |
Target: 5'- uGCUGCCggGGCUCCuggggugcugccgggGCUCCugggGUGCUGCCGg -3' miRNA: 3'- -CGGUGG--CCGGGG---------------UGGGG----UACGACGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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