Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13449 | 3' | -56.3 | NC_003409.1 | + | 77660 | 0.66 | 0.881983 |
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Target: 5'- uGCAgUCCGacUCUGCC-CCUCUacccaGGGUGg -3' miRNA: 3'- -CGU-AGGC--AGACGGaGGAGAcg---CUCAC- -5' |
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| 13449 | 3' | -56.3 | NC_003409.1 | + | 106459 | 0.67 | 0.835777 |
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Target: 5'- ---cCCGUgCUGaCUUCUCUGCGAGg- -3' miRNA: 3'- cguaGGCA-GACgGAGGAGACGCUCac -5' |
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| 13449 | 3' | -56.3 | NC_003409.1 | + | 44876 | 0.67 | 0.835777 |
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Target: 5'- cGCAUCUG-CUGCCUCCcUUUGCc---- -3' miRNA: 3'- -CGUAGGCaGACGGAGG-AGACGcucac -5' |
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| 13449 | 3' | -56.3 | NC_003409.1 | + | 104935 | 0.67 | 0.82738 |
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Target: 5'- cGUAUcCCG-CUGCCUgCUCUgGCGGGc- -3' miRNA: 3'- -CGUA-GGCaGACGGAgGAGA-CGCUCac -5' |
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| 13449 | 3' | -56.3 | NC_003409.1 | + | 105476 | 0.73 | 0.521731 |
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Target: 5'- cCGUCCG-CUGCUUUCUCgggGUGGGUGu -3' miRNA: 3'- cGUAGGCaGACGGAGGAGa--CGCUCAC- -5' |
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| 13449 | 3' | -56.3 | NC_003409.1 | + | 90326 | 0.73 | 0.492455 |
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Target: 5'- uGCAUCCGUgUcGCCUCCgggCUGUGAuGUu -3' miRNA: 3'- -CGUAGGCAgA-CGGAGGa--GACGCU-CAc -5' |
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| 13449 | 3' | -56.3 | NC_003409.1 | + | 115762 | 0.98 | 0.013378 |
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Target: 5'- uGCAUCC-UCUGCCUCCUCUGCGAGUGc -3' miRNA: 3'- -CGUAGGcAGACGGAGGAGACGCUCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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