Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1345 | 3' | -56.7 | NC_001335.1 | + | 18029 | 0.66 | 0.630357 |
Target: 5'- -gUCGAGCUgguUCUGCagCGCcUCU-CGGCc -3' miRNA: 3'- ugAGCUCGG---AGACG--GCGaAGAuGCCG- -5' |
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1345 | 3' | -56.7 | NC_001335.1 | + | 10459 | 0.66 | 0.630357 |
Target: 5'- -gUUGAGCC-CUGCgGCUUCcGCugagcuguaGGCg -3' miRNA: 3'- ugAGCUCGGaGACGgCGAAGaUG---------CCG- -5' |
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1345 | 3' | -56.7 | NC_001335.1 | + | 2353 | 0.66 | 0.608398 |
Target: 5'- cCUCGA-CCUCgcugGcCCGCUgggCUuCGGCg -3' miRNA: 3'- uGAGCUcGGAGa---C-GGCGAa--GAuGCCG- -5' |
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1345 | 3' | -56.7 | NC_001335.1 | + | 15161 | 0.67 | 0.575635 |
Target: 5'- cGCUucgCGGGCCUUcugGCCcaGCUUCagcaGCGGCu -3' miRNA: 3'- -UGA---GCUCGGAGa--CGG--CGAAGa---UGCCG- -5' |
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1345 | 3' | -56.7 | NC_001335.1 | + | 24769 | 0.67 | 0.575635 |
Target: 5'- -aUCGAGCUUC-GCCGCaaggacaUCguggacgACGGCa -3' miRNA: 3'- ugAGCUCGGAGaCGGCGa------AGa------UGCCG- -5' |
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1345 | 3' | -56.7 | NC_001335.1 | + | 38597 | 0.67 | 0.564795 |
Target: 5'- aACaaGuGCCUgUGCCcCUUCcACGGCg -3' miRNA: 3'- -UGagCuCGGAgACGGcGAAGaUGCCG- -5' |
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1345 | 3' | -56.7 | NC_001335.1 | + | 2942 | 0.67 | 0.532631 |
Target: 5'- -gUCGGGCCaUCUGCCag-UCgguCGGCu -3' miRNA: 3'- ugAGCUCGG-AGACGGcgaAGau-GCCG- -5' |
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1345 | 3' | -56.7 | NC_001335.1 | + | 5892 | 0.68 | 0.511556 |
Target: 5'- cGCUCGGGCCuggUCUGCCuGCcgaUACcGCa -3' miRNA: 3'- -UGAGCUCGG---AGACGG-CGaagAUGcCG- -5' |
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1345 | 3' | -56.7 | NC_001335.1 | + | 32947 | 0.68 | 0.511556 |
Target: 5'- gGCUCcuGAGCCUgaGaCCGCgccgUCggcgACGGUg -3' miRNA: 3'- -UGAG--CUCGGAgaC-GGCGa---AGa---UGCCG- -5' |
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1345 | 3' | -56.7 | NC_001335.1 | + | 19463 | 0.68 | 0.511556 |
Target: 5'- uGCUUGAGCCguucCUGCuCGCgcUUGCaGCg -3' miRNA: 3'- -UGAGCUCGGa---GACG-GCGaaGAUGcCG- -5' |
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1345 | 3' | -56.7 | NC_001335.1 | + | 16543 | 0.68 | 0.501148 |
Target: 5'- --aCGGGCCUCgGCCcuguGCUcgCUAcCGGCa -3' miRNA: 3'- ugaGCUCGGAGaCGG----CGAa-GAU-GCCG- -5' |
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1345 | 3' | -56.7 | NC_001335.1 | + | 48849 | 0.69 | 0.440856 |
Target: 5'- cCUCGAGgCUUgcGCCGCccgCUACGGg -3' miRNA: 3'- uGAGCUCgGAGa-CGGCGaa-GAUGCCg -5' |
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1345 | 3' | -56.7 | NC_001335.1 | + | 49392 | 0.69 | 0.431209 |
Target: 5'- gGCggCGAGg-UUUGCCGCUaCUACGGCc -3' miRNA: 3'- -UGa-GCUCggAGACGGCGAaGAUGCCG- -5' |
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1345 | 3' | -56.7 | NC_001335.1 | + | 26173 | 0.7 | 0.358739 |
Target: 5'- cCUgGAGCCcgCUGCCGCUUgaCUGCGc- -3' miRNA: 3'- uGAgCUCGGa-GACGGCGAA--GAUGCcg -5' |
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1345 | 3' | -56.7 | NC_001335.1 | + | 9935 | 0.71 | 0.310248 |
Target: 5'- gGCUUGAGCC---GCCGCguacuccUCUGCGGUc -3' miRNA: 3'- -UGAGCUCGGagaCGGCGa------AGAUGCCG- -5' |
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1345 | 3' | -56.7 | NC_001335.1 | + | 31771 | 0.73 | 0.266909 |
Target: 5'- aGCUCGcgcuGCCUCgcggGCCuGCUUgcGCGGCa -3' miRNA: 3'- -UGAGCu---CGGAGa---CGG-CGAAgaUGCCG- -5' |
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1345 | 3' | -56.7 | NC_001335.1 | + | 33999 | 0.73 | 0.26018 |
Target: 5'- cCUUGAGCaCUCgguggGCuCGCUUCUGCuugGGCa -3' miRNA: 3'- uGAGCUCG-GAGa----CG-GCGAAGAUG---CCG- -5' |
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1345 | 3' | -56.7 | NC_001335.1 | + | 9887 | 0.74 | 0.216901 |
Target: 5'- gACUCGAugGCCggCUGUgGCUUCgGCGGUg -3' miRNA: 3'- -UGAGCU--CGGa-GACGgCGAAGaUGCCG- -5' |
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1345 | 3' | -56.7 | NC_001335.1 | + | 43051 | 0.75 | 0.189885 |
Target: 5'- cUUCGAGCCUCUGCCugaacCUgacaaaUGCGGCu -3' miRNA: 3'- uGAGCUCGGAGACGGc----GAag----AUGCCG- -5' |
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1345 | 3' | -56.7 | NC_001335.1 | + | 11014 | 1.12 | 0.00041 |
Target: 5'- cACUCGAGCCUCUGCCGCUUCUACGGCg -3' miRNA: 3'- -UGAGCUCGGAGACGGCGAAGAUGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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