Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1345 | 5' | -62.5 | NC_001335.1 | + | 331 | 0.68 | 0.233698 |
Target: 5'- gCGCCGCCUGuGCGGCgcucuauagagagggGCucuuAGCC-CCu -3' miRNA: 3'- aGCGGUGGGCuCGCCGa--------------CG----UCGGaGG- -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 16144 | 0.69 | 0.200219 |
Target: 5'- aUCGCCuagaucUCCGAGCccccGGCUGCcgccgaggAGaCCUCCg -3' miRNA: 3'- -AGCGGu-----GGGCUCG----CCGACG--------UC-GGAGG- -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 12457 | 0.7 | 0.195146 |
Target: 5'- aUCGCCACU--GGCGGCUggaaGguGCC-CCg -3' miRNA: 3'- -AGCGGUGGgcUCGCCGA----CguCGGaGG- -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 43194 | 0.7 | 0.190186 |
Target: 5'- gUCGUCACCCG-GCGaGUUGcCGGUCUUg -3' miRNA: 3'- -AGCGGUGGGCuCGC-CGAC-GUCGGAGg -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 24357 | 0.7 | 0.185337 |
Target: 5'- gUCGCaccuGCCUGAGCGGCgaacGUAGCg-CCg -3' miRNA: 3'- -AGCGg---UGGGCUCGCCGa---CGUCGgaGG- -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 6526 | 0.7 | 0.184858 |
Target: 5'- uUCGCCaagucgacccaagGCCCGAGCuGGCcGCAuuuguacaaccGCUUCCc -3' miRNA: 3'- -AGCGG-------------UGGGCUCG-CCGaCGU-----------CGGAGG- -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 45192 | 0.71 | 0.15848 |
Target: 5'- cUCGaCCACCUG-GCGG--GCGGUCUCCu -3' miRNA: 3'- -AGC-GGUGGGCuCGCCgaCGUCGGAGG- -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 13858 | 0.72 | 0.12953 |
Target: 5'- gUGCCGCCgauGGCGGCUcgggugaggauguaaGUGGCCUCCg -3' miRNA: 3'- aGCGGUGGgc-UCGCCGA---------------CGUCGGAGG- -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 1220 | 0.73 | 0.106108 |
Target: 5'- aCGCCGCCCGAuccgGgGGCUccaGC-GCCUCUg -3' miRNA: 3'- aGCGGUGGGCU----CgCCGA---CGuCGGAGG- -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 20577 | 0.75 | 0.080732 |
Target: 5'- cCGCCucugggcggucuGCUCGAuGCGGUguUGCAGCCUCUg -3' miRNA: 3'- aGCGG------------UGGGCU-CGCCG--ACGUCGGAGG- -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 10980 | 1.11 | 0.000136 |
Target: 5'- uUCGCCACCCGAGCGGCUGCAGCCUCCu -3' miRNA: 3'- -AGCGGUGGGCUCGCCGACGUCGGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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