Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13453 | 5' | -50.1 | NC_003409.1 | + | 108814 | 0.68 | 0.950561 |
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Target: 5'- uGGAGuGuGGAUGGUgauauggucuccugGGCCUGGc -3' miRNA: 3'- gCCUCuC-CUUACCA--------------CCGGACCa -5' |
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| 13453 | 5' | -50.1 | NC_003409.1 | + | 117611 | 1.03 | 0.014084 |
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Target: 5'- gCGGAGAGGAAUGGUGGCCUGGUu -3' miRNA: 3'- -GCCUCUCCUUACCACCGGACCA- -5' |
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| 13453 | 5' | -50.1 | NC_003409.1 | + | 117736 | 0.7 | 0.894036 |
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Target: 5'- uCGGuGGGGGAgGGUGcCCUGGUu -3' miRNA: 3'- -GCCuCUCCUUaCCACcGGACCA- -5' |
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| 13453 | 5' | -50.1 | NC_003409.1 | + | 118301 | 0.73 | 0.780612 |
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Target: 5'- cCGGGGAGGAGgcuggGGUGcGCCgcgccgGGUu -3' miRNA: 3'- -GCCUCUCCUUa----CCAC-CGGa-----CCA- -5' |
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| 13453 | 5' | -50.1 | NC_003409.1 | + | 129137 | 0.67 | 0.97364 |
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Target: 5'- gCGGGGAGGAGggGGUGGg--GGa -3' miRNA: 3'- -GCCUCUCCUUa-CCACCggaCCa -5' |
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| 13453 | 5' | -50.1 | NC_003409.1 | + | 133308 | 0.68 | 0.96016 |
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Target: 5'- aGGGcguugccAGGAGUGGUGGCgaUGGUg -3' miRNA: 3'- gCCUc------UCCUUACCACCGg-ACCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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