Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13455 | 3' | -59.5 | NC_003409.1 | + | 131079 | 0.66 | 0.734424 |
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Target: 5'- uGGCGUagcuGGCCUGGCAgCCUGUUCUgAg -3' miRNA: 3'- -UCGCAc---CUGGAUCGUaGGGCGAGGgU- -5' |
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| 13455 | 3' | -59.5 | NC_003409.1 | + | 66658 | 0.66 | 0.71485 |
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Target: 5'- uGGCGUGGACUUuGUGUCCa-CUUCCc -3' miRNA: 3'- -UCGCACCUGGAuCGUAGGgcGAGGGu -5' |
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| 13455 | 3' | -59.5 | NC_003409.1 | + | 81241 | 0.67 | 0.684993 |
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Target: 5'- cGGCcgaGGACgaAGCGUCCgGCUUCCc -3' miRNA: 3'- -UCGca-CCUGgaUCGUAGGgCGAGGGu -5' |
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| 13455 | 3' | -59.5 | NC_003409.1 | + | 68074 | 0.67 | 0.674943 |
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Target: 5'- uGGCGgcauacacugGGACCggGGCAUacggacuuucaCCUGCUUCCAg -3' miRNA: 3'- -UCGCa---------CCUGGa-UCGUA-----------GGGCGAGGGU- -5' |
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| 13455 | 3' | -59.5 | NC_003409.1 | + | 33443 | 0.68 | 0.604089 |
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Target: 5'- ----aGGGCCUGGaCGUCCUGCUUUCAc -3' miRNA: 3'- ucgcaCCUGGAUC-GUAGGGCGAGGGU- -5' |
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| 13455 | 3' | -59.5 | NC_003409.1 | + | 53238 | 0.68 | 0.583916 |
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Target: 5'- gGGCGUGGACUacaUGGCGUUCUG-UCCgGg -3' miRNA: 3'- -UCGCACCUGG---AUCGUAGGGCgAGGgU- -5' |
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| 13455 | 3' | -59.5 | NC_003409.1 | + | 98352 | 0.68 | 0.573878 |
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Target: 5'- uGGCGUGGuacaaACgaAGCAUCCUccccCUCCCAu -3' miRNA: 3'- -UCGCACC-----UGgaUCGUAGGGc---GAGGGU- -5' |
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| 13455 | 3' | -59.5 | NC_003409.1 | + | 118760 | 0.97 | 0.00742 |
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Target: 5'- gAG-GUGGACCUAGCAUCCCGCUCCCAu -3' miRNA: 3'- -UCgCACCUGGAUCGUAGGGCGAGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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