Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1346 | 3' | -56.1 | NC_001335.1 | + | 38764 | 0.7 | 0.455473 |
Target: 5'- cUCGACgCAGCggagaCCGG-CGaGGGCUUCGCc -3' miRNA: 3'- -GGCUG-GUCGa----GGUCuGC-CCUGAAGUG- -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 43212 | 0.7 | 0.445701 |
Target: 5'- aUGACCuaugacguggaGGCUCCgaAGACGGGcgacuACUUCAUg -3' miRNA: 3'- gGCUGG-----------UCGAGG--UCUGCCC-----UGAAGUG- -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 47707 | 0.73 | 0.292288 |
Target: 5'- aCCGAggcUCGGCUCCAGA-GGGACcacaucaucgUCACg -3' miRNA: 3'- -GGCU---GGUCGAGGUCUgCCCUGa---------AGUG- -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 40384 | 0.66 | 0.667431 |
Target: 5'- gCUGAUCAaCUcuccCCAGGCGGGGCgcgCAUg -3' miRNA: 3'- -GGCUGGUcGA----GGUCUGCCCUGaa-GUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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