Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13460 | 3' | -42.2 | NC_003409.1 | + | 121424 | 1.07 | 0.060773 |
Target: 5'- gUGUCACAUUCUGUGACCGCGAc -3' miRNA: 3'- -ACAGUGUAAGACACUGGCGCU- -5' |
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13460 | 3' | -42.2 | NC_003409.1 | + | 121624 | 0.68 | 0.99999 |
Target: 5'- -aUCACAUUCUGagGACgGCa- -3' miRNA: 3'- acAGUGUAAGACa-CUGgCGcu -5' |
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13460 | 3' | -42.2 | NC_003409.1 | + | 123499 | 0.68 | 0.999987 |
Target: 5'- cUGUCAgGUUCucccaucgacgacgcUG-GGCCGCGAg -3' miRNA: 3'- -ACAGUgUAAG---------------ACaCUGGCGCU- -5' |
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13460 | 3' | -42.2 | NC_003409.1 | + | 131466 | 0.66 | 1 |
Target: 5'- cUGUCuCGUUCUGcaaaACCGUGAc -3' miRNA: 3'- -ACAGuGUAAGACac--UGGCGCU- -5' |
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13460 | 3' | -42.2 | NC_003409.1 | + | 134565 | 0.67 | 0.999999 |
Target: 5'- ---gACAacCUGUGACUGCGAc -3' miRNA: 3'- acagUGUaaGACACUGGCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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