miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
13463 3' -58.1 NC_003409.1 + 35180 0.66 0.80775
Target:  5'- aGGgGAGGGUUCUggGGCCGG-CUACUu -3'
miRNA:   3'- aCCaCUCCUAAGG--CUGGCCgGGUGAu -5'
13463 3' -58.1 NC_003409.1 + 31494 0.66 0.80775
Target:  5'- cGGUGGGGAacaUCUGGCC--CCCACa- -3'
miRNA:   3'- aCCACUCCUa--AGGCUGGccGGGUGau -5'
13463 3' -58.1 NC_003409.1 + 82254 0.66 0.771323
Target:  5'- gGGUGGGGGgauggUCCG-CCGGgCgACg- -3'
miRNA:   3'- aCCACUCCUa----AGGCuGGCCgGgUGau -5'
13463 3' -58.1 NC_003409.1 + 108763 0.67 0.742671
Target:  5'- aUGGUGgccaacAGGAggCCGGCCuGCuCCGCUu -3'
miRNA:   3'- -ACCAC------UCCUaaGGCUGGcCG-GGUGAu -5'
13463 3' -58.1 NC_003409.1 + 133363 0.67 0.739753
Target:  5'- gGGUGGGGAcUCCcGCucccugcagcaucuCGGCCUGCUGu -3'
miRNA:   3'- aCCACUCCUaAGGcUG--------------GCCGGGUGAU- -5'
13463 3' -58.1 NC_003409.1 + 106786 0.7 0.550881
Target:  5'- cUGGUGAGGcgUUC-ACCgggGGCCCGCa- -3'
miRNA:   3'- -ACCACUCCuaAGGcUGG---CCGGGUGau -5'
13463 3' -58.1 NC_003409.1 + 130917 1.06 0.002268
Target:  5'- aUGGUGAGGAUUCCGACCGGCCCACUAc -3'
miRNA:   3'- -ACCACUCCUAAGGCUGGCCGGGUGAU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.