Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1348 | 3' | -58.4 | NC_001335.1 | + | 3557 | 0.66 | 0.568506 |
Target: 5'- cGUUUCCGGcuccgacGGCugCgUCCAgagcgucuuggauGUCGCCu -3' miRNA: 3'- cCAAAGGCC-------CCGugGaAGGU-------------CGGCGG- -5' |
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1348 | 3' | -58.4 | NC_001335.1 | + | 32987 | 0.66 | 0.560043 |
Target: 5'- ---aUCCGuGGaGCACCgccccugcCCAGCCcgaGCCg -3' miRNA: 3'- ccaaAGGC-CC-CGUGGaa------GGUCGG---CGG- -5' |
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1348 | 3' | -58.4 | NC_001335.1 | + | 44403 | 0.66 | 0.560043 |
Target: 5'- ---cUCgGGGGacgUACCUgaaCCcGCCGCCg -3' miRNA: 3'- ccaaAGgCCCC---GUGGAa--GGuCGGCGG- -5' |
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1348 | 3' | -58.4 | NC_001335.1 | + | 14901 | 0.66 | 0.518326 |
Target: 5'- aGGUUUCCuuggcGGGCcugGCCUcccccUCCucCCGCCa -3' miRNA: 3'- -CCAAAGGc----CCCG---UGGA-----AGGucGGCGG- -5' |
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1348 | 3' | -58.4 | NC_001335.1 | + | 7038 | 0.67 | 0.497929 |
Target: 5'- cGGUaacCCGGucGGCaaGCCgcgUUCAGCCGCg -3' miRNA: 3'- -CCAaa-GGCC--CCG--UGGa--AGGUCGGCGg -5' |
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1348 | 3' | -58.4 | NC_001335.1 | + | 26580 | 0.67 | 0.49188 |
Target: 5'- cGGaucagCCGGGGaccgaGCCgaacggccuugagCCGGCCGUCg -3' miRNA: 3'- -CCaaa--GGCCCCg----UGGaa-----------GGUCGGCGG- -5' |
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1348 | 3' | -58.4 | NC_001335.1 | + | 37082 | 0.67 | 0.468034 |
Target: 5'- cGUaUgCGGGGCcCCUUCaccgaGGUCGCUg -3' miRNA: 3'- cCAaAgGCCCCGuGGAAGg----UCGGCGG- -5' |
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1348 | 3' | -58.4 | NC_001335.1 | + | 11815 | 0.68 | 0.420369 |
Target: 5'- uGGUggacCCGGacGGCACCggcUCCAacGCCGaCCu -3' miRNA: 3'- -CCAaa--GGCC--CCGUGGa--AGGU--CGGC-GG- -5' |
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1348 | 3' | -58.4 | NC_001335.1 | + | 35382 | 0.69 | 0.40214 |
Target: 5'- aGGUguucCCGGaGCuguucCCgcaCCAGCCGCCg -3' miRNA: 3'- -CCAaa--GGCCcCGu----GGaa-GGUCGGCGG- -5' |
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1348 | 3' | -58.4 | NC_001335.1 | + | 1168 | 0.69 | 0.3749 |
Target: 5'- cGGga-CCGGGGcCGCCUUuacuuccCCGGCCuCCc -3' miRNA: 3'- -CCaaaGGCCCC-GUGGAA-------GGUCGGcGG- -5' |
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1348 | 3' | -58.4 | NC_001335.1 | + | 1305 | 0.69 | 0.367229 |
Target: 5'- cGGUgacgccUCCGGuaccGGCGCUgccgUUCCcuuGCCGCCu -3' miRNA: 3'- -CCAa-----AGGCC----CCGUGG----AAGGu--CGGCGG- -5' |
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1348 | 3' | -58.4 | NC_001335.1 | + | 24168 | 0.69 | 0.358832 |
Target: 5'- ----aCCuGGGCACCagCCccGCCGCCg -3' miRNA: 3'- ccaaaGGcCCCGUGGaaGGu-CGGCGG- -5' |
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1348 | 3' | -58.4 | NC_001335.1 | + | 7577 | 0.7 | 0.350571 |
Target: 5'- ----aCCGGGGUgucggccgGCgCUUCCAGCgCGUCg -3' miRNA: 3'- ccaaaGGCCCCG--------UG-GAAGGUCG-GCGG- -5' |
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1348 | 3' | -58.4 | NC_001335.1 | + | 30047 | 0.7 | 0.326607 |
Target: 5'- aGGg--CCGGGGguCCa-CCAGCuuCGCCa -3' miRNA: 3'- -CCaaaGGCCCCguGGaaGGUCG--GCGG- -5' |
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1348 | 3' | -58.4 | NC_001335.1 | + | 39283 | 0.71 | 0.275496 |
Target: 5'- aGGUcUCCGGGGUACCgggUgCAGaaCCGUa -3' miRNA: 3'- -CCAaAGGCCCCGUGGa--AgGUC--GGCGg -5' |
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1348 | 3' | -58.4 | NC_001335.1 | + | 1354 | 0.74 | 0.192693 |
Target: 5'- gGGgugUUGGGGCugCUgCCAccGCCGCCg -3' miRNA: 3'- -CCaaaGGCCCCGugGAaGGU--CGGCGG- -5' |
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1348 | 3' | -58.4 | NC_001335.1 | + | 12543 | 1.14 | 0.00021 |
Target: 5'- cGGUUUCCGGGGCACCUUCCAGCCGCCa -3' miRNA: 3'- -CCAAAGGCCCCGUGGAAGGUCGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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