Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1348 | 5' | -53.2 | NC_001335.1 | + | 6983 | 0.66 | 0.788925 |
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Target: 5'- gCGcCUGCUUGGCGgAACUCUgUGGc- -3' miRNA: 3'- aGUuGACGAACCGC-UUGAGGaGCCuu -5' |
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| 1348 | 5' | -53.2 | NC_001335.1 | + | 34800 | 0.66 | 0.778681 |
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Target: 5'- uUCAACUGCUggguagcuggcUGGCccgaGAAgaUCUCGGAc -3' miRNA: 3'- -AGUUGACGA-----------ACCG----CUUgaGGAGCCUu -5' |
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| 1348 | 5' | -53.2 | NC_001335.1 | + | 35980 | 0.67 | 0.725276 |
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Target: 5'- aCAGCU-CUUGGaUGAuCUCCUCGGc- -3' miRNA: 3'- aGUUGAcGAACC-GCUuGAGGAGCCuu -5' |
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| 1348 | 5' | -53.2 | NC_001335.1 | + | 1597 | 0.67 | 0.704245 |
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Target: 5'- -gAAgUGCUUGGUGcgguggacgaucucCUCCUCGGAGa -3' miRNA: 3'- agUUgACGAACCGCuu------------GAGGAGCCUU- -5' |
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| 1348 | 5' | -53.2 | NC_001335.1 | + | 49880 | 0.67 | 0.69194 |
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Target: 5'- aUCGACg---UGGCGcGCUUCUUGGAAg -3' miRNA: 3'- -AGUUGacgaACCGCuUGAGGAGCCUU- -5' |
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| 1348 | 5' | -53.2 | NC_001335.1 | + | 8560 | 0.68 | 0.623925 |
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Target: 5'- gUAGCUGgUguugcUGGCGAGaUCCUUGGAAg -3' miRNA: 3'- aGUUGACgA-----ACCGCUUgAGGAGCCUU- -5' |
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| 1348 | 5' | -53.2 | NC_001335.1 | + | 43552 | 0.69 | 0.589867 |
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Target: 5'- cCGAUgucCUUGGCGuACUCCUCGcGGAa -3' miRNA: 3'- aGUUGac-GAACCGCuUGAGGAGC-CUU- -5' |
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| 1348 | 5' | -53.2 | NC_001335.1 | + | 33037 | 0.71 | 0.490664 |
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Target: 5'- aCuGCUGCUUGGUGAuucGCUCgUaCGGAGc -3' miRNA: 3'- aGuUGACGAACCGCU---UGAGgA-GCCUU- -5' |
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| 1348 | 5' | -53.2 | NC_001335.1 | + | 12509 | 1.07 | 0.001667 |
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Target: 5'- aUCAACUGCUUGGCGAACUCCUCGGAAa -3' miRNA: 3'- -AGUUGACGAACCGCUUGAGGAGCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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