Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13485 | 5' | -48 | NC_003417.1 | + | 7865 | 0.66 | 0.714584 |
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Target: 5'- aGGCGUGCAAGAucugcauUGUCuaucaccccgggAACGUGUgcugGCu -3' miRNA: 3'- gCUGCAUGUUCU-------GCAG------------UUGUACGa---CG- -5' |
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| 13485 | 5' | -48 | NC_003417.1 | + | 449 | 0.66 | 0.690454 |
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Target: 5'- uGAC-UGCAGGuCGUCuuCuucGCUGCg -3' miRNA: 3'- gCUGcAUGUUCuGCAGuuGua-CGACG- -5' |
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| 13485 | 5' | -48 | NC_003417.1 | + | 1527 | 0.67 | 0.625926 |
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Target: 5'- gGACGUagGCGAGGCcagagCAGCAgagagggaaGCUGCu -3' miRNA: 3'- gCUGCA--UGUUCUGca---GUUGUa--------CGACG- -5' |
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| 13485 | 5' | -48 | NC_003417.1 | + | 10720 | 0.67 | 0.600051 |
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Target: 5'- uGACGUGCAcuGUGUCuACAUGCacGCa -3' miRNA: 3'- gCUGCAUGUucUGCAGuUGUACGa-CG- -5' |
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| 13485 | 5' | -48 | NC_003417.1 | + | 2795 | 0.68 | 0.574328 |
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Target: 5'- uCGugG-ACAcGAgGUCAugACAgcUGCUGCa -3' miRNA: 3'- -GCugCaUGUuCUgCAGU--UGU--ACGACG- -5' |
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| 13485 | 5' | -48 | NC_003417.1 | + | 3490 | 0.7 | 0.439963 |
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Target: 5'- aGAU-UGCAGguGACGUCGAUcgGCUGUg -3' miRNA: 3'- gCUGcAUGUU--CUGCAGUUGuaCGACG- -5' |
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| 13485 | 5' | -48 | NC_003417.1 | + | 2447 | 0.86 | 0.041885 |
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Target: 5'- uCGACGUACAAGACGUC-ACAguCUGCu -3' miRNA: 3'- -GCUGCAUGUUCUGCAGuUGUacGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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