Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1349 | 3' | -54.6 | NC_001335.1 | + | 6797 | 0.66 | 0.729407 |
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Target: 5'- cGUCUGGCGacgUUGAUC--GCGCUCu- -3' miRNA: 3'- cUAGGCCGCg--AACUAGuaCGCGAGcu -5' |
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| 1349 | 3' | -54.6 | NC_001335.1 | + | 31554 | 0.67 | 0.649111 |
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Target: 5'- cGAUCCuagacacccaagagGGUGUccUGAUCAUcGCGUUCGAu -3' miRNA: 3'- -CUAGG--------------CCGCGa-ACUAGUA-CGCGAGCU- -5' |
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| 1349 | 3' | -54.6 | NC_001335.1 | + | 16031 | 0.67 | 0.641298 |
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Target: 5'- -cUUCGGU-CUUGAgcugcaucaUCAUGCGCUUGAa -3' miRNA: 3'- cuAGGCCGcGAACU---------AGUACGCGAGCU- -5' |
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| 1349 | 3' | -54.6 | NC_001335.1 | + | 22117 | 0.69 | 0.520092 |
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Target: 5'- --aCCGGCGCUgugcaggcGAUCAUgaacGCGCUCc- -3' miRNA: 3'- cuaGGCCGCGAa-------CUAGUA----CGCGAGcu -5' |
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| 1349 | 3' | -54.6 | NC_001335.1 | + | 36174 | 0.69 | 0.520092 |
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Target: 5'- uGAUCUGGCGC--GAaCGUGCGUUCu- -3' miRNA: 3'- -CUAGGCCGCGaaCUaGUACGCGAGcu -5' |
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| 1349 | 3' | -54.6 | NC_001335.1 | + | 42344 | 0.7 | 0.498878 |
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Target: 5'- --aCCGGCGCgagccgCGUGCGuCUCGGc -3' miRNA: 3'- cuaGGCCGCGaacua-GUACGC-GAGCU- -5' |
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| 1349 | 3' | -54.6 | NC_001335.1 | + | 12682 | 0.7 | 0.477029 |
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Target: 5'- --gCCGGCGCUcGAUCA-GCGCcaucacuUCGGg -3' miRNA: 3'- cuaGGCCGCGAaCUAGUaCGCG-------AGCU- -5' |
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| 1349 | 3' | -54.6 | NC_001335.1 | + | 16258 | 0.7 | 0.467811 |
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Target: 5'- --gCCGGCGCUgGcgUAgucGCGCUCGGc -3' miRNA: 3'- cuaGGCCGCGAaCuaGUa--CGCGAGCU- -5' |
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| 1349 | 3' | -54.6 | NC_001335.1 | + | 12718 | 1.08 | 0.001106 |
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Target: 5'- gGAUCCGGCGCUUGAUCAUGCGCUCGAu -3' miRNA: 3'- -CUAGGCCGCGAACUAGUACGCGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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