Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1350 | 3' | -56.6 | NC_001335.1 | + | 5647 | 0.66 | 0.665936 |
Target: 5'- aCGGCACGuuguccaccagcCCGGuGGAUCAGg-UCa- -3' miRNA: 3'- gGCCGUGU------------GGCCuCCUAGUCgaAGca -5' |
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1350 | 3' | -56.6 | NC_001335.1 | + | 13933 | 0.66 | 0.644208 |
Target: 5'- -gGGuCugACCGGAGGAUCGGg----- -3' miRNA: 3'- ggCC-GugUGGCCUCCUAGUCgaagca -5' |
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1350 | 3' | -56.6 | NC_001335.1 | + | 8927 | 0.66 | 0.633323 |
Target: 5'- cCCGGUugAuCCGGGGGucgaucUCGGCgugcaUUCGg -3' miRNA: 3'- -GGCCGugU-GGCCUCCu-----AGUCG-----AAGCa -5' |
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1350 | 3' | -56.6 | NC_001335.1 | + | 25364 | 0.66 | 0.622436 |
Target: 5'- cUCaGCGCAgCGGAGGAUCcaAGCcUCa- -3' miRNA: 3'- -GGcCGUGUgGCCUCCUAG--UCGaAGca -5' |
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1350 | 3' | -56.6 | NC_001335.1 | + | 26063 | 0.67 | 0.589864 |
Target: 5'- gCCGGC-CACCgauacgacgguGGAcaGGAUCAGCagCGa -3' miRNA: 3'- -GGCCGuGUGG-----------CCU--CCUAGUCGaaGCa -5' |
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1350 | 3' | -56.6 | NC_001335.1 | + | 37514 | 0.67 | 0.589864 |
Target: 5'- uCCGGUugauaGCC-GAGGAUCGGCUU-GUg -3' miRNA: 3'- -GGCCGug---UGGcCUCCUAGUCGAAgCA- -5' |
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1350 | 3' | -56.6 | NC_001335.1 | + | 12019 | 0.67 | 0.579064 |
Target: 5'- uCCuuCGCGCCGuuGAGGAUCGGCU-CGg -3' miRNA: 3'- -GGccGUGUGGC--CUCCUAGUCGAaGCa -5' |
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1350 | 3' | -56.6 | NC_001335.1 | + | 46984 | 0.67 | 0.568308 |
Target: 5'- gCCGGUACGCCGGAaGA--GGCUgUGUu -3' miRNA: 3'- -GGCCGUGUGGCCUcCUagUCGAaGCA- -5' |
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1350 | 3' | -56.6 | NC_001335.1 | + | 29010 | 0.7 | 0.425977 |
Target: 5'- gCCGGCGgcUGCCGGuGGA-CAGCUcgCGg -3' miRNA: 3'- -GGCCGU--GUGGCCuCCUaGUCGAa-GCa -5' |
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1350 | 3' | -56.6 | NC_001335.1 | + | 23314 | 0.71 | 0.346419 |
Target: 5'- aCGGagGCACCGGAGcuucGA-CAGCUUCGg -3' miRNA: 3'- gGCCg-UGUGGCCUC----CUaGUCGAAGCa -5' |
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1350 | 3' | -56.6 | NC_001335.1 | + | 41480 | 0.72 | 0.322372 |
Target: 5'- gCCGGCcccgGCACCGGGaaGUCGGCgUUCGUc -3' miRNA: 3'- -GGCCG----UGUGGCCUccUAGUCG-AAGCA- -5' |
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1350 | 3' | -56.6 | NC_001335.1 | + | 30036 | 0.72 | 0.322372 |
Target: 5'- gCCGGCcucgaaGgGCCGGGGGuccacCAGCUUCGc -3' miRNA: 3'- -GGCCG------UgUGGCCUCCua---GUCGAAGCa -5' |
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1350 | 3' | -56.6 | NC_001335.1 | + | 5662 | 0.73 | 0.257816 |
Target: 5'- gCCGGCGCACCGGAGac-UAGUaUCGa -3' miRNA: 3'- -GGCCGUGUGGCCUCcuaGUCGaAGCa -5' |
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1350 | 3' | -56.6 | NC_001335.1 | + | 13092 | 1.1 | 0.000651 |
Target: 5'- aCCGGCACACCGGAGGAUCAGCUUCGUg -3' miRNA: 3'- -GGCCGUGUGGCCUCCUAGUCGAAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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