Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1350 | 5' | -54.8 | NC_001335.1 | + | 34929 | 0.66 | 0.716621 |
Target: 5'- ---aGCGUcaGGCuuGGUCCuGGCGUAGg -3' miRNA: 3'- uuugCGCAa-CUGggCUAGG-CCGCAUU- -5' |
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1350 | 5' | -54.8 | NC_001335.1 | + | 26220 | 0.66 | 0.683572 |
Target: 5'- gAGGCGCGgguucUGGCCgCGAgacagguacUUCGGCGUGc -3' miRNA: 3'- -UUUGCGCa----ACUGG-GCU---------AGGCCGCAUu -5' |
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1350 | 5' | -54.8 | NC_001335.1 | + | 36565 | 0.66 | 0.661265 |
Target: 5'- cAACGCGaUGAUgCGGUUCGGCa--- -3' miRNA: 3'- uUUGCGCaACUGgGCUAGGCCGcauu -5' |
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1350 | 5' | -54.8 | NC_001335.1 | + | 5314 | 0.66 | 0.661265 |
Target: 5'- cGAAuCGUGUUGGCCgCGAagggcUUgGGCGUGAa -3' miRNA: 3'- -UUU-GCGCAACUGG-GCU-----AGgCCGCAUU- -5' |
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1350 | 5' | -54.8 | NC_001335.1 | + | 46891 | 0.66 | 0.661265 |
Target: 5'- cGAACGCuUUcGCCUGAacacagccucuUCCGGCGUAc -3' miRNA: 3'- -UUUGCGcAAcUGGGCU-----------AGGCCGCAUu -5' |
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1350 | 5' | -54.8 | NC_001335.1 | + | 37782 | 0.67 | 0.650063 |
Target: 5'- aGAACGCuGUUcGCgCCGGUCaCGGUGUAc -3' miRNA: 3'- -UUUGCG-CAAcUG-GGCUAG-GCCGCAUu -5' |
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1350 | 5' | -54.8 | NC_001335.1 | + | 4268 | 0.67 | 0.638843 |
Target: 5'- cGAGgGCugaaccgGACCCGuGUCCGGUGUAGg -3' miRNA: 3'- -UUUgCGcaa----CUGGGC-UAGGCCGCAUU- -5' |
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1350 | 5' | -54.8 | NC_001335.1 | + | 40654 | 0.68 | 0.582857 |
Target: 5'- -cACGCGgagaGGCCCaggaucgccGAUCCGGCGa-- -3' miRNA: 3'- uuUGCGCaa--CUGGG---------CUAGGCCGCauu -5' |
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1350 | 5' | -54.8 | NC_001335.1 | + | 5174 | 0.68 | 0.538771 |
Target: 5'- --cCGCGUcGACCagCGAUCUGGCGa-- -3' miRNA: 3'- uuuGCGCAaCUGG--GCUAGGCCGCauu -5' |
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1350 | 5' | -54.8 | NC_001335.1 | + | 9237 | 0.72 | 0.326632 |
Target: 5'- cGAUGCGUaccUGGCCCgGAUCCuGGCGUu- -3' miRNA: 3'- uUUGCGCA---ACUGGG-CUAGG-CCGCAuu -5' |
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1350 | 5' | -54.8 | NC_001335.1 | + | 13056 | 1.03 | 0.002275 |
Target: 5'- cAAACGCGUUGACCCGAUCCGGCGUAAc -3' miRNA: 3'- -UUUGCGCAACUGGGCUAGGCCGCAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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