Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1351 | 5' | -52.8 | NC_001335.1 | + | 50806 | 0.66 | 0.849425 |
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Target: 5'- aUGGCGGU-GCCUaccgugaucUCUuccaacUGGCGUUcagCCa -3' miRNA: 3'- gACCGCCAcCGGA---------AGA------ACUGCAAa--GG- -5' |
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| 1351 | 5' | -52.8 | NC_001335.1 | + | 51848 | 0.66 | 0.840622 |
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Target: 5'- gCUGGUcucGGUGGCaucgCUUGACaGUcgCCc -3' miRNA: 3'- -GACCG---CCACCGgaa-GAACUG-CAaaGG- -5' |
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| 1351 | 5' | -52.8 | NC_001335.1 | + | 25132 | 0.68 | 0.706113 |
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Target: 5'- gCUGGCGGcuGCCUUCUUcgcccgguccugugGAgGggUCCa -3' miRNA: 3'- -GACCGCCacCGGAAGAA--------------CUgCaaAGG- -5' |
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| 1351 | 5' | -52.8 | NC_001335.1 | + | 22594 | 0.7 | 0.620333 |
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Target: 5'- gCUGGCGGcgUGGCCggCggUGACGacUUCg -3' miRNA: 3'- -GACCGCC--ACCGGaaGa-ACUGCaaAGG- -5' |
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| 1351 | 5' | -52.8 | NC_001335.1 | + | 46447 | 0.71 | 0.575707 |
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Target: 5'- -cGGCGGUcaGGCgUUCcUUGACGg--CCu -3' miRNA: 3'- gaCCGCCA--CCGgAAG-AACUGCaaaGG- -5' |
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| 1351 | 5' | -52.8 | NC_001335.1 | + | 29942 | 0.72 | 0.499752 |
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Target: 5'- -cGGCGGcuagcuUGGCCaugagcUUCUUGACGUUcucggccuggUCCu -3' miRNA: 3'- gaCCGCC------ACCGG------AAGAACUGCAA----------AGG- -5' |
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| 1351 | 5' | -52.8 | NC_001335.1 | + | 13145 | 1.11 | 0.001224 |
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Target: 5'- uCUGGCGGUGGCCUUCUUGACGUUUCCc -3' miRNA: 3'- -GACCGCCACCGGAAGAACUGCAAAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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