Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1353 | 5' | -60.1 | NC_001335.1 | + | 16478 | 0.66 | 0.461215 |
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Target: 5'- aGGuuGGGGGugAUCGcggucagcaucGGGCCgaGCUGCUGg -3' miRNA: 3'- aCUggCCUCC--UAGC-----------CCCGG--CGAUGAC- -5' |
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| 1353 | 5' | -60.1 | NC_001335.1 | + | 42016 | 0.66 | 0.451578 |
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Target: 5'- cGGgUGGAucucGGAUCGGccaGGCCGCaaacgGCUGa -3' miRNA: 3'- aCUgGCCU----CCUAGCC---CCGGCGa----UGAC- -5' |
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| 1353 | 5' | -60.1 | NC_001335.1 | + | 1181 | 0.67 | 0.405127 |
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Target: 5'- cGugcuCCGGuuccGGGAcCGGGGCCGCcuuUACUu -3' miRNA: 3'- aCu---GGCC----UCCUaGCCCCGGCG---AUGAc -5' |
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| 1353 | 5' | -60.1 | NC_001335.1 | + | 34727 | 0.67 | 0.370205 |
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Target: 5'- aGGgCGGGGGAgUCGGGGCgaaCUACUc -3' miRNA: 3'- aCUgGCCUCCU-AGCCCCGgc-GAUGAc -5' |
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| 1353 | 5' | -60.1 | NC_001335.1 | + | 50219 | 0.69 | 0.292257 |
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Target: 5'- uUGACCGGAGGG-Ca-GGCCGaUGCUGu -3' miRNA: 3'- -ACUGGCCUCCUaGccCCGGCgAUGAC- -5' |
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| 1353 | 5' | -60.1 | NC_001335.1 | + | 1358 | 0.74 | 0.127409 |
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Target: 5'- -cACCGGGGuGUUGGGGCUGCUGCc- -3' miRNA: 3'- acUGGCCUCcUAGCCCCGGCGAUGac -5' |
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| 1353 | 5' | -60.1 | NC_001335.1 | + | 13929 | 1.07 | 0.000469 |
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Target: 5'- cUGACCGGAGGAUCGGGGCCGCUACUGc -3' miRNA: 3'- -ACUGGCCUCCUAGCCCCGGCGAUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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