Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13531 | 3' | -48.7 | NC_003436.1 | + | 6270 | 0.66 | 0.876943 |
Target: 5'- -cCCCAACGUacugGUAUUAUAuuGCGUa -3' miRNA: 3'- guGGGUUGCGgaaaCGUAAUAU--CGCG- -5' |
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13531 | 3' | -48.7 | NC_003436.1 | + | 9390 | 0.66 | 0.859201 |
Target: 5'- uGCCC-ACGCCa-UGU--UAUAGCGUc -3' miRNA: 3'- gUGGGuUGCGGaaACGuaAUAUCGCG- -5' |
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13531 | 3' | -48.7 | NC_003436.1 | + | 14269 | 0.66 | 0.849886 |
Target: 5'- cCACCCAcugugcgugcACGuUCUUUGCcacuaAUAGCGUa -3' miRNA: 3'- -GUGGGU----------UGC-GGAAACGuaa--UAUCGCG- -5' |
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13531 | 3' | -48.7 | NC_003436.1 | + | 6358 | 1.14 | 0.000797 |
Target: 5'- aCACCCAACGCCUUUGCAUUAUAGCGCa -3' miRNA: 3'- -GUGGGUUGCGGAAACGUAAUAUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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