Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1354 | 5' | -57 | NC_001335.1 | + | 49873 | 0.66 | 0.609024 |
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Target: 5'- uGGCGCgCUUCUUGGaagacaaGggcaucaaCAUCGCCUAc -3' miRNA: 3'- gCUGCG-GAAGAGCCg------Ca-------GUAGCGGAU- -5' |
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| 1354 | 5' | -57 | NC_001335.1 | + | 37886 | 0.66 | 0.587235 |
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Target: 5'- gGGCGaacUCcauacCGGCGUCGUCGCCg- -3' miRNA: 3'- gCUGCggaAGa----GCCGCAGUAGCGGau -5' |
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| 1354 | 5' | -57 | NC_001335.1 | + | 39121 | 0.66 | 0.587235 |
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Target: 5'- uCGugGCCcuggauaCUUGGCGUC-UCGCgUGa -3' miRNA: 3'- -GCugCGGaa-----GAGCCGCAGuAGCGgAU- -5' |
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| 1354 | 5' | -57 | NC_001335.1 | + | 24795 | 0.67 | 0.565595 |
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Target: 5'- aCGAUGUCcagCUCcucaGGCGUCAgCGCCUc -3' miRNA: 3'- -GCUGCGGaa-GAG----CCGCAGUaGCGGAu -5' |
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| 1354 | 5' | -57 | NC_001335.1 | + | 24287 | 0.67 | 0.544167 |
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Target: 5'- aGACGCCaUCU-GGUacuUCAUCGCCa- -3' miRNA: 3'- gCUGCGGaAGAgCCGc--AGUAGCGGau -5' |
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| 1354 | 5' | -57 | NC_001335.1 | + | 7490 | 0.68 | 0.45181 |
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Target: 5'- -cAUGCCUUCUCGGCGaccgucUCggUGCCg- -3' miRNA: 3'- gcUGCGGAAGAGCCGC------AGuaGCGGau -5' |
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| 1354 | 5' | -57 | NC_001335.1 | + | 5893 | 0.69 | 0.432446 |
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Target: 5'- gGACGCCaga-CGGUGUCGUCaGCCa- -3' miRNA: 3'- gCUGCGGaagaGCCGCAGUAG-CGGau -5' |
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| 1354 | 5' | -57 | NC_001335.1 | + | 47572 | 0.69 | 0.413574 |
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Target: 5'- aCGAgGCUUUCUCGGCGggCAgaGCCc- -3' miRNA: 3'- -GCUgCGGAAGAGCCGCa-GUagCGGau -5' |
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| 1354 | 5' | -57 | NC_001335.1 | + | 51844 | 0.7 | 0.386241 |
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Target: 5'- uGuCGCUggUCUCGGUGgCAUCGCUUGa -3' miRNA: 3'- gCuGCGGa-AGAGCCGCaGUAGCGGAU- -5' |
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| 1354 | 5' | -57 | NC_001335.1 | + | 16163 | 1.07 | 0.000815 |
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Target: 5'- aCGACGCCUUCUCGGCGUCAUCGCCUAg -3' miRNA: 3'- -GCUGCGGAAGAGCCGCAGUAGCGGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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