Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13540 | 3' | -57.1 | NC_003444.1 | + | 6064 | 0.66 | 0.51889 |
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Target: 5'- --aCCGGGUauuGAGGCU--CCACGCCa -3' miRNA: 3'- cgcGGCCCGcu-UUCUGAagGGUGCGG- -5' |
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| 13540 | 3' | -57.1 | NC_003444.1 | + | 15157 | 0.66 | 0.497863 |
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Target: 5'- uGCGCCGGaGCGugucgcuGACgUCCa--GCCu -3' miRNA: 3'- -CGCGGCC-CGCuuu----CUGaAGGgugCGG- -5' |
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| 13540 | 3' | -57.1 | NC_003444.1 | + | 847 | 0.67 | 0.467067 |
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Target: 5'- -gGCUGGGUcaauAAAGGC-UCCCggcuGCGCCg -3' miRNA: 3'- cgCGGCCCGc---UUUCUGaAGGG----UGCGG- -5' |
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| 13540 | 3' | -57.1 | NC_003444.1 | + | 29753 | 0.67 | 0.457021 |
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Target: 5'- uGUGCCGcguauuacacGGCGGAAGACaa--CGCGCUg -3' miRNA: 3'- -CGCGGC----------CCGCUUUCUGaaggGUGCGG- -5' |
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| 13540 | 3' | -57.1 | NC_003444.1 | + | 11434 | 0.67 | 0.447091 |
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Target: 5'- cCGCCGGau-AAGGugUguaucCCCGCGCCg -3' miRNA: 3'- cGCGGCCcgcUUUCugAa----GGGUGCGG- -5' |
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| 13540 | 3' | -57.1 | NC_003444.1 | + | 9785 | 0.68 | 0.426639 |
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Target: 5'- gGCGuugaucaCCGGuGuCGAcAGGCUuucUCCCGCGCUg -3' miRNA: 3'- -CGC-------GGCC-C-GCUuUCUGA---AGGGUGCGG- -5' |
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| 13540 | 3' | -57.1 | NC_003444.1 | + | 13137 | 0.69 | 0.38116 |
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Target: 5'- cUGgCGGGUGAuaucacACUgaaaUCCCGCGCCa -3' miRNA: 3'- cGCgGCCCGCUuuc---UGA----AGGGUGCGG- -5' |
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| 13540 | 3' | -57.1 | NC_003444.1 | + | 12769 | 0.76 | 0.124537 |
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Target: 5'- uGCGCCGGGUGAAagcagaucugaaugcGGAUaUCaacaCGCGCCu -3' miRNA: 3'- -CGCGGCCCGCUU---------------UCUGaAGg---GUGCGG- -5' |
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| 13540 | 3' | -57.1 | NC_003444.1 | + | 1076 | 1.14 | 0.000208 |
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Target: 5'- cGCGCCGGGCGAAAGACUUCCCACGCCu -3' miRNA: 3'- -CGCGGCCCGCUUUCUGAAGGGUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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