Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13540 | 5' | -54.8 | NC_003444.1 | + | 24468 | 0.66 | 0.644189 |
Target: 5'- uUGGUuuucguguucaucacACCGGAAgcgacuuCACUgagaaCUGCGCCg -3' miRNA: 3'- cACCG---------------UGGCCUUau-----GUGAa----GGCGCGG- -5' |
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13540 | 5' | -54.8 | NC_003444.1 | + | 13143 | 0.66 | 0.62829 |
Target: 5'- -cGGcCACUGGcggguGAUaucACACUgaaaucCCGCGCCa -3' miRNA: 3'- caCC-GUGGCC-----UUA---UGUGAa-----GGCGCGG- -5' |
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13540 | 5' | -54.8 | NC_003444.1 | + | 33455 | 0.66 | 0.605595 |
Target: 5'- cUGGUACCaGcuucuggACAUgaugCCGCGCCa -3' miRNA: 3'- cACCGUGGcCuua----UGUGaa--GGCGCGG- -5' |
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13540 | 5' | -54.8 | NC_003444.1 | + | 14650 | 0.67 | 0.57176 |
Target: 5'- -aGcGCACCGcuGGAUcCGCUgacCCGUGCCg -3' miRNA: 3'- caC-CGUGGC--CUUAuGUGAa--GGCGCGG- -5' |
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13540 | 5' | -54.8 | NC_003444.1 | + | 29234 | 0.67 | 0.547233 |
Target: 5'- uGUGGUucACCGuugugaagaGGAUACACgcagacauuucCCGCGCCu -3' miRNA: 3'- -CACCG--UGGC---------CUUAUGUGaa---------GGCGCGG- -5' |
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13540 | 5' | -54.8 | NC_003444.1 | + | 13019 | 0.68 | 0.527417 |
Target: 5'- aGUGGaaugGCUGGAcaucgGUGCGCaUCgGUGCCg -3' miRNA: 3'- -CACCg---UGGCCU-----UAUGUGaAGgCGCGG- -5' |
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13540 | 5' | -54.8 | NC_003444.1 | + | 24861 | 0.68 | 0.527417 |
Target: 5'- -aGGCACCGGGAgGCAC--CCG-GCa -3' miRNA: 3'- caCCGUGGCCUUaUGUGaaGGCgCGg -5' |
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13540 | 5' | -54.8 | NC_003444.1 | + | 22966 | 0.68 | 0.505722 |
Target: 5'- aGUGGUGCgGGAauguccuggcaAUACGCUUCUGuUGCa -3' miRNA: 3'- -CACCGUGgCCU-----------UAUGUGAAGGC-GCGg -5' |
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13540 | 5' | -54.8 | NC_003444.1 | + | 30416 | 0.68 | 0.505722 |
Target: 5'- cGUGGC-CCGGGGgaucACGCUUcauaCCGCGgUg -3' miRNA: 3'- -CACCGuGGCCUUa---UGUGAA----GGCGCgG- -5' |
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13540 | 5' | -54.8 | NC_003444.1 | + | 12970 | 0.68 | 0.505722 |
Target: 5'- aUGGCauuacGCCGGAuaauGUcCGCgUCaCGCGCCg -3' miRNA: 3'- cACCG-----UGGCCU----UAuGUGaAG-GCGCGG- -5' |
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13540 | 5' | -54.8 | NC_003444.1 | + | 11369 | 0.68 | 0.495019 |
Target: 5'- -cGGCGCgGGGAUACACaccuuaUCCG-GCg -3' miRNA: 3'- caCCGUGgCCUUAUGUGa-----AGGCgCGg -5' |
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13540 | 5' | -54.8 | NC_003444.1 | + | 24799 | 0.69 | 0.443171 |
Target: 5'- uGUGGUGCCGG-GUGC-CUcCCgGUGCCu -3' miRNA: 3'- -CACCGUGGCCuUAUGuGAaGG-CGCGG- -5' |
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13540 | 5' | -54.8 | NC_003444.1 | + | 36915 | 0.7 | 0.394524 |
Target: 5'- uGUGGUcaaCGGuuuuCGCUUCaCGCGCCa -3' miRNA: 3'- -CACCGug-GCCuuauGUGAAG-GCGCGG- -5' |
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13540 | 5' | -54.8 | NC_003444.1 | + | 32506 | 0.71 | 0.349469 |
Target: 5'- -cGGCGacuCCGGAuc-CACUUUCaGCGCCa -3' miRNA: 3'- caCCGU---GGCCUuauGUGAAGG-CGCGG- -5' |
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13540 | 5' | -54.8 | NC_003444.1 | + | 11472 | 0.74 | 0.235793 |
Target: 5'- uGUGGCACCGGAGgcauuguUugACUgagCCGgaucuacCGCCg -3' miRNA: 3'- -CACCGUGGCCUU-------AugUGAa--GGC-------GCGG- -5' |
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13540 | 5' | -54.8 | NC_003444.1 | + | 1038 | 1.13 | 0.000354 |
Target: 5'- cGUGGCACCGGAAUACACUUCCGCGCCg -3' miRNA: 3'- -CACCGUGGCCUUAUGUGAAGGCGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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