Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13543 | 5' | -51.9 | NC_003444.1 | + | 35976 | 0.66 | 0.811063 |
Target: 5'- cCUGACG-AAUACUgccGCGGUCAAUcaUCg -3' miRNA: 3'- -GACUGCaUUAUGGgc-CGCCAGUUG--AG- -5' |
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13543 | 5' | -51.9 | NC_003444.1 | + | 15388 | 0.67 | 0.719548 |
Target: 5'- gCUGAauaacacuCCCGGCGGUCAcCUUc -3' miRNA: 3'- -GACUgcauuau-GGGCCGCCAGUuGAG- -5' |
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13543 | 5' | -51.9 | NC_003444.1 | + | 6184 | 0.68 | 0.680943 |
Target: 5'- -aGugGUGAUccCCCGGCGG-UAACg- -3' miRNA: 3'- gaCugCAUUAu-GGGCCGCCaGUUGag -5' |
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13543 | 5' | -51.9 | NC_003444.1 | + | 30412 | 0.68 | 0.646369 |
Target: 5'- gUGGCGUG--GCCCGGgGGaUCAcGCUUc -3' miRNA: 3'- gACUGCAUuaUGGGCCgCC-AGU-UGAG- -5' |
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13543 | 5' | -51.9 | NC_003444.1 | + | 3480 | 0.98 | 0.008407 |
Target: 5'- cCUGACGUAAUACCCGGCGGcUAACUCa -3' miRNA: 3'- -GACUGCAUUAUGGGCCGCCaGUUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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