Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13544 | 5' | -51.4 | NC_003444.1 | + | 975 | 0.66 | 0.782906 |
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Target: 5'- -aCCGGCAGAGauuGCCGAUUUgccguucuuaCGGggGa -3' miRNA: 3'- cgGGUCGUCUUg--UGGUUGAA----------GCCuuC- -5' |
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| 13544 | 5' | -51.4 | NC_003444.1 | + | 30579 | 0.67 | 0.728408 |
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Target: 5'- cGCCCAGCGuGAACACguACUggCGa--- -3' miRNA: 3'- -CGGGUCGU-CUUGUGguUGAa-GCcuuc -5' |
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| 13544 | 5' | -51.4 | NC_003444.1 | + | 4814 | 0.68 | 0.71713 |
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Target: 5'- -gCCGGUgaAGAagACACCGAUUUCGGuauGGg -3' miRNA: 3'- cgGGUCG--UCU--UGUGGUUGAAGCCu--UC- -5' |
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| 13544 | 5' | -51.4 | NC_003444.1 | + | 481 | 0.68 | 0.710317 |
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Target: 5'- cCCCAGCAGc-CGCCAGCgUCugauuggccuggcggGGAAGa -3' miRNA: 3'- cGGGUCGUCuuGUGGUUGaAG---------------CCUUC- -5' |
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| 13544 | 5' | -51.4 | NC_003444.1 | + | 1029 | 0.68 | 0.698893 |
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Target: 5'- cGCCCGGCGcGAcugauguguaaacgcACACCcauGCUgaCGGAAGc -3' miRNA: 3'- -CGGGUCGU-CU---------------UGUGGu--UGAa-GCCUUC- -5' |
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| 13544 | 5' | -51.4 | NC_003444.1 | + | 865 | 0.69 | 0.636283 |
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Target: 5'- uCCCGGCu--GCGCCG-CUUCaGGGAGg -3' miRNA: 3'- cGGGUCGucuUGUGGUuGAAG-CCUUC- -5' |
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| 13544 | 5' | -51.4 | NC_003444.1 | + | 10140 | 0.7 | 0.566618 |
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Target: 5'- cGCaCCAGCAucGCAUCAugUUCuGGAAa -3' miRNA: 3'- -CG-GGUCGUcuUGUGGUugAAG-CCUUc -5' |
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| 13544 | 5' | -51.4 | NC_003444.1 | + | 17179 | 0.71 | 0.532453 |
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Target: 5'- gGCgCGGCAGGGCAgCCGACUguaUCGGc-- -3' miRNA: 3'- -CGgGUCGUCUUGU-GGUUGA---AGCCuuc -5' |
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| 13544 | 5' | -51.4 | NC_003444.1 | + | 4838 | 1.11 | 0.001005 |
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Target: 5'- cGCCCAGCAGAACACCAACUUCGGAAGu -3' miRNA: 3'- -CGGGUCGUCUUGUGGUUGAAGCCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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