Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13546 | 3' | -46 | NC_003444.1 | + | 11692 | 0.66 | 0.986572 |
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Target: 5'- uUUCGC-GGUGuACCGuuuAAGGUCGAAg -3' miRNA: 3'- cGAGCGaCUAC-UGGCu--UUUUAGCUUg -5' |
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| 13546 | 3' | -46 | NC_003444.1 | + | 9203 | 0.66 | 0.983923 |
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Target: 5'- cCUCGuCUGAUGagaugaccaucacuGCCGAGcuggCGAACg -3' miRNA: 3'- cGAGC-GACUAC--------------UGGCUUuuuaGCUUG- -5' |
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| 13546 | 3' | -46 | NC_003444.1 | + | 35785 | 0.68 | 0.963668 |
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Target: 5'- aGUUUGCgagGGUGAUCGGAGuAAUCaguGAACa -3' miRNA: 3'- -CGAGCGa--CUACUGGCUUU-UUAG---CUUG- -5' |
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| 13546 | 3' | -46 | NC_003444.1 | + | 7806 | 0.7 | 0.893103 |
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Target: 5'- gGUUaCGCUGAcggUGACCGggGAAgcaacCGAAa -3' miRNA: 3'- -CGA-GCGACU---ACUGGCuuUUUa----GCUUg -5' |
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| 13546 | 3' | -46 | NC_003444.1 | + | 36362 | 0.71 | 0.876787 |
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Target: 5'- uGC-CGCUGcUGACCGGuugcGGAAguacgCGAACa -3' miRNA: 3'- -CGaGCGACuACUGGCU----UUUUa----GCUUG- -5' |
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| 13546 | 3' | -46 | NC_003444.1 | + | 8217 | 0.79 | 0.441322 |
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Target: 5'- --aCGCUGGUGACCGAGA---UGAACg -3' miRNA: 3'- cgaGCGACUACUGGCUUUuuaGCUUG- -5' |
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| 13546 | 3' | -46 | NC_003444.1 | + | 7232 | 1.14 | 0.003047 |
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Target: 5'- aGCUCGCUGAUGACCGAAAAAUCGAACu -3' miRNA: 3'- -CGAGCGACUACUGGCUUUUUAGCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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