Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13548 | 3' | -56.3 | NC_003444.1 | + | 7520 | 0.66 | 0.512667 |
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Target: 5'- --aACGCGCCGgauaucacccGCUG-CUUCaCgGCGCa -3' miRNA: 3'- guaUGCGCGGU----------CGACuGAAG-GgCGUG- -5' |
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| 13548 | 3' | -56.3 | NC_003444.1 | + | 23034 | 0.66 | 0.501884 |
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Target: 5'- -cUGCgGCaCCAGCUGGCUgcaaagaaCCGCAUg -3' miRNA: 3'- guAUG-CGcGGUCGACUGAag------GGCGUG- -5' |
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| 13548 | 3' | -56.3 | NC_003444.1 | + | 13753 | 0.66 | 0.501884 |
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Target: 5'- uGUAuUGCGCguGCUGACUUugaagCCCGacaACg -3' miRNA: 3'- gUAU-GCGCGguCGACUGAA-----GGGCg--UG- -5' |
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| 13548 | 3' | -56.3 | NC_003444.1 | + | 8258 | 0.67 | 0.474334 |
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Target: 5'- cUAUGCGCGUCAGCUGuauggucauguguauACggcaaagaCCgGCACa -3' miRNA: 3'- -GUAUGCGCGGUCGAC---------------UGaa------GGgCGUG- -5' |
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| 13548 | 3' | -56.3 | NC_003444.1 | + | 27633 | 0.67 | 0.449592 |
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Target: 5'- --gACGCGCCAGCgu-CUUuuuuaucgUCCGCAUu -3' miRNA: 3'- guaUGCGCGGUCGacuGAA--------GGGCGUG- -5' |
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| 13548 | 3' | -56.3 | NC_003444.1 | + | 1084 | 0.68 | 0.419694 |
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Target: 5'- aCAUcaguCGCGCCgGGCgaaaGACUUCCCaCGCc -3' miRNA: 3'- -GUAu---GCGCGG-UCGa---CUGAAGGGcGUG- -5' |
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| 13548 | 3' | -56.3 | NC_003444.1 | + | 15281 | 0.75 | 0.142025 |
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Target: 5'- gCAUGuCGCGCCAGCcagGACUcaUCGCACa -3' miRNA: 3'- -GUAU-GCGCGGUCGa--CUGAagGGCGUG- -5' |
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| 13548 | 3' | -56.3 | NC_003444.1 | + | 7762 | 1.08 | 0.000458 |
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Target: 5'- cCAUACGCGCCAGCUGACUUCCCGCACc -3' miRNA: 3'- -GUAUGCGCGGUCGACUGAAGGGCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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