Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1355 | 3' | -63.6 | NC_001335.1 | + | 47446 | 0.7 | 0.152423 |
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Target: 5'- --uCAGGuuGGCCAGCAGugCauuGGCCAc -3' miRNA: 3'- gccGUCUggCCGGUCGUCugG---CCGGU- -5' |
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| 1355 | 3' | -63.6 | NC_001335.1 | + | 13196 | 0.69 | 0.173678 |
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Target: 5'- -aGCGGACCaGGCCgucgaauaccGGCAucCCGGCCAu -3' miRNA: 3'- gcCGUCUGG-CCGG----------UCGUcuGGCCGGU- -5' |
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| 1355 | 3' | -63.6 | NC_001335.1 | + | 32145 | 0.69 | 0.173678 |
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Target: 5'- cCGGUAGcaguuCCGGCCcGCGaACuCGGCCAg -3' miRNA: 3'- -GCCGUCu----GGCCGGuCGUcUG-GCCGGU- -5' |
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| 1355 | 3' | -63.6 | NC_001335.1 | + | 4091 | 0.69 | 0.178234 |
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Target: 5'- aGGCAG-CCGGCggcgaugcaaAGCAGcUCGGCCGc -3' miRNA: 3'- gCCGUCuGGCCGg---------UCGUCuGGCCGGU- -5' |
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| 1355 | 3' | -63.6 | NC_001335.1 | + | 2320 | 0.69 | 0.182897 |
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Target: 5'- uCGGCaaggagugagcuGGGCCGGCU--CAGGCCGGCg- -3' miRNA: 3'- -GCCG------------UCUGGCCGGucGUCUGGCCGgu -5' |
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| 1355 | 3' | -63.6 | NC_001335.1 | + | 11374 | 0.69 | 0.182897 |
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Target: 5'- uCGGCGugaacGACCccaaGGUCGcGCAGACCGGCg- -3' miRNA: 3'- -GCCGU-----CUGG----CCGGU-CGUCUGGCCGgu -5' |
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| 1355 | 3' | -63.6 | NC_001335.1 | + | 11513 | 0.76 | 0.054306 |
|
Target: 5'- gGGCAcGACCGGCCAGaagaucccgcacUGGACCGGCg- -3' miRNA: 3'- gCCGU-CUGGCCGGUC------------GUCUGGCCGgu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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