Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13551 | 5' | -56.6 | NC_003444.1 | + | 32495 | 0.66 | 0.519653 |
Target: 5'- cGCCGCCgcuggguuaaugaaaAGUACaC-GCGCuGGGUuAAGACa -3' miRNA: 3'- -CGGCGG---------------UCGUG-GuCGCG-CCCA-UUUUG- -5' |
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13551 | 5' | -56.6 | NC_003444.1 | + | 12649 | 0.66 | 0.504572 |
Target: 5'- gGCCaGCCAGUGCuCuGCGCaGGUGu--- -3' miRNA: 3'- -CGG-CGGUCGUG-GuCGCGcCCAUuuug -5' |
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13551 | 5' | -56.6 | NC_003444.1 | + | 12754 | 0.66 | 0.483366 |
Target: 5'- cGCUGUUucuGGCGCU-GCGCcGGGUGAAAg -3' miRNA: 3'- -CGGCGG---UCGUGGuCGCG-CCCAUUUUg -5' |
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13551 | 5' | -56.6 | NC_003444.1 | + | 14313 | 0.66 | 0.472925 |
Target: 5'- aCCGCCugaaagGGCugCAGa-CGGGUGAggUg -3' miRNA: 3'- cGGCGG------UCGugGUCgcGCCCAUUuuG- -5' |
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13551 | 5' | -56.6 | NC_003444.1 | + | 169 | 0.66 | 0.472925 |
Target: 5'- uCCgGCCAGCGCCuuGCGCGcguuGGcuUGGGGCg -3' miRNA: 3'- cGG-CGGUCGUGGu-CGCGC----CC--AUUUUG- -5' |
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13551 | 5' | -56.6 | NC_003444.1 | + | 8200 | 0.67 | 0.452392 |
Target: 5'- uCCGCCAuuGCAgCCAccGCGCcGGUGAGAa -3' miRNA: 3'- cGGCGGU--CGU-GGU--CGCGcCCAUUUUg -5' |
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13551 | 5' | -56.6 | NC_003444.1 | + | 15714 | 0.67 | 0.452392 |
Target: 5'- -gUGUCGGUAuguCCAuGCGCGGGUAAuGCc -3' miRNA: 3'- cgGCGGUCGU---GGU-CGCGCCCAUUuUG- -5' |
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13551 | 5' | -56.6 | NC_003444.1 | + | 8074 | 0.67 | 0.421557 |
Target: 5'- uGCUGCCAGCgggcguacagauuGCCguGGCGaCGGGgaccgccGGAACg -3' miRNA: 3'- -CGGCGGUCG-------------UGG--UCGC-GCCCa------UUUUG- -5' |
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13551 | 5' | -56.6 | NC_003444.1 | + | 12093 | 0.68 | 0.366533 |
Target: 5'- cGCCGUCGccGCACCugauGUG-GGGUAAGAg -3' miRNA: 3'- -CGGCGGU--CGUGGu---CGCgCCCAUUUUg -5' |
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13551 | 5' | -56.6 | NC_003444.1 | + | 7936 | 0.69 | 0.332144 |
Target: 5'- cGCCGUUGGuCACCGGagccugcaCGCGGGUAcgucccacauAGACa -3' miRNA: 3'- -CGGCGGUC-GUGGUC--------GCGCCCAU----------UUUG- -5' |
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13551 | 5' | -56.6 | NC_003444.1 | + | 35780 | 0.69 | 0.323926 |
Target: 5'- aGCCGCCGGacguUACCAGCuucuuucacGCuGGUGGGAUg -3' miRNA: 3'- -CGGCGGUC----GUGGUCG---------CGcCCAUUUUG- -5' |
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13551 | 5' | -56.6 | NC_003444.1 | + | 488 | 0.7 | 0.285119 |
Target: 5'- aGCCGCCAGCGuCUgauuGGCcugGCGGGgaagaagAAAGCc -3' miRNA: 3'- -CGGCGGUCGU-GG----UCG---CGCCCa------UUUUG- -5' |
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13551 | 5' | -56.6 | NC_003444.1 | + | 16676 | 0.71 | 0.270656 |
Target: 5'- aCCGCgCAGaaggauaaguUCGGCGCGGGUAAGAa -3' miRNA: 3'- cGGCG-GUCgu--------GGUCGCGCCCAUUUUg -5' |
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13551 | 5' | -56.6 | NC_003444.1 | + | 436 | 0.71 | 0.250076 |
Target: 5'- aCCGCCcauuGCACCGGUGauGGUGuuGCc -3' miRNA: 3'- cGGCGGu---CGUGGUCGCgcCCAUuuUG- -5' |
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13551 | 5' | -56.6 | NC_003444.1 | + | 30642 | 0.71 | 0.243509 |
Target: 5'- uGUCGCCAGUACguGUucacgcuggGCGGGaAAGGCa -3' miRNA: 3'- -CGGCGGUCGUGguCG---------CGCCCaUUUUG- -5' |
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13551 | 5' | -56.6 | NC_003444.1 | + | 3468 | 0.75 | 0.140003 |
Target: 5'- cCCGgCGGCuaACuCAGCGCGGGUAAAcaaGCg -3' miRNA: 3'- cGGCgGUCG--UG-GUCGCGCCCAUUU---UG- -5' |
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13551 | 5' | -56.6 | NC_003444.1 | + | 6390 | 0.8 | 0.063611 |
Target: 5'- cCCGCCGGaCACgGGCGCGGuUAAAGCg -3' miRNA: 3'- cGGCGGUC-GUGgUCGCGCCcAUUUUG- -5' |
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13551 | 5' | -56.6 | NC_003444.1 | + | 12917 | 1.11 | 0.000292 |
Target: 5'- cGCCGCCAGCACCAGCGCGGGUAAAACc -3' miRNA: 3'- -CGGCGGUCGUGGUCGCGCCCAUUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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