Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13552 | 3' | -52.7 | NC_003444.1 | + | 6464 | 0.66 | 0.767558 |
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Target: 5'- cGGUGCcGACCGcgcAUGGcGCAUCa---- -3' miRNA: 3'- -CCACGaCUGGCu--UGCC-CGUAGcauug -5' |
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| 13552 | 3' | -52.7 | NC_003444.1 | + | 27166 | 0.66 | 0.756899 |
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Target: 5'- uGUGCa-GCUc--CGGGCGUCGUGGCg -3' miRNA: 3'- cCACGacUGGcuuGCCCGUAGCAUUG- -5' |
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| 13552 | 3' | -52.7 | NC_003444.1 | + | 14376 | 0.66 | 0.756899 |
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Target: 5'- --cGCUGAcCCGGGaGGGgAUCGUggUg -3' miRNA: 3'- ccaCGACU-GGCUUgCCCgUAGCAuuG- -5' |
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| 13552 | 3' | -52.7 | NC_003444.1 | + | 8814 | 0.67 | 0.711889 |
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Target: 5'- gGGUGacaaucgcCUGACCGGgaccaaaGCGGGUaccGUCGcUGGCa -3' miRNA: 3'- -CCAC--------GACUGGCU-------UGCCCG---UAGC-AUUG- -5' |
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| 13552 | 3' | -52.7 | NC_003444.1 | + | 722 | 0.68 | 0.62186 |
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Target: 5'- aGGacCUGGCUGAACGGGUcgCGa--- -3' miRNA: 3'- -CCacGACUGGCUUGCCCGuaGCauug -5' |
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| 13552 | 3' | -52.7 | NC_003444.1 | + | 13762 | 0.7 | 0.498377 |
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Target: 5'- cGUGCUGAcuuugaagcCCGAcaACGGGCggCGcgGACg -3' miRNA: 3'- cCACGACU---------GGCU--UGCCCGuaGCa-UUG- -5' |
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| 13552 | 3' | -52.7 | NC_003444.1 | + | 8072 | 0.71 | 0.435656 |
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Target: 5'- aGUGCUG-CC-AGCGGGCGUacagauugcCGUGGCg -3' miRNA: 3'- cCACGACuGGcUUGCCCGUA---------GCAUUG- -5' |
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| 13552 | 3' | -52.7 | NC_003444.1 | + | 12868 | 0.72 | 0.403159 |
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Target: 5'- uGGUGCUGGCgGcgaccugguuugauAACGcGGCG-CGUGACg -3' miRNA: 3'- -CCACGACUGgC--------------UUGC-CCGUaGCAUUG- -5' |
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| 13552 | 3' | -52.7 | NC_003444.1 | + | 27798 | 0.73 | 0.359618 |
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Target: 5'- uGGUGgUGaACUGAACGGGguUggCGUAACc -3' miRNA: 3'- -CCACgAC-UGGCUUGCCCguA--GCAUUG- -5' |
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| 13552 | 3' | -52.7 | NC_003444.1 | + | 13282 | 1.13 | 0.000578 |
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Target: 5'- cGGUGCUGACCGAACGGGCAUCGUAACg -3' miRNA: 3'- -CCACGACUGGCUUGCCCGUAGCAUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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