Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13552 | 5' | -59 | NC_003444.1 | + | 15170 | 0.66 | 0.418335 |
Target: 5'- aCGUCUUCAUCCAugcGCCGGagcguGUCGCUg -3' miRNA: 3'- aGCGGAGGUGGGU---CGGCCac---UAGUGG- -5' |
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13552 | 5' | -59 | NC_003444.1 | + | 12936 | 0.66 | 0.408978 |
Target: 5'- aCGCaUCCcgGCUUuguGCCGGUGAUC-CCu -3' miRNA: 3'- aGCGgAGG--UGGGu--CGGCCACUAGuGG- -5' |
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13552 | 5' | -59 | NC_003444.1 | + | 2478 | 0.66 | 0.390661 |
Target: 5'- aUC-CUUCCACCCcgauaaCCGGUGAU-GCCg -3' miRNA: 3'- -AGcGGAGGUGGGuc----GGCCACUAgUGG- -5' |
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13552 | 5' | -59 | NC_003444.1 | + | 10188 | 0.67 | 0.381706 |
Target: 5'- gUCGCUUuacccacaCCACCCAgaauguuuucuGCCGG-GAUCcCCu -3' miRNA: 3'- -AGCGGA--------GGUGGGU-----------CGGCCaCUAGuGG- -5' |
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13552 | 5' | -59 | NC_003444.1 | + | 26889 | 0.67 | 0.381706 |
Target: 5'- aUUGCCcau-CCCAGCgGGaaaaucUGAUCGCCa -3' miRNA: 3'- -AGCGGagguGGGUCGgCC------ACUAGUGG- -5' |
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13552 | 5' | -59 | NC_003444.1 | + | 6099 | 0.67 | 0.359072 |
Target: 5'- aCGCCaUCACCCauuacAucaccauucguuaccGCCGGggGAUCACCa -3' miRNA: 3'- aGCGGaGGUGGG-----U---------------CGGCCa-CUAGUGG- -5' |
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13552 | 5' | -59 | NC_003444.1 | + | 6122 | 0.67 | 0.347279 |
Target: 5'- -gGUUUCCugCCAGgUGGUGG-CACUg -3' miRNA: 3'- agCGGAGGugGGUCgGCCACUaGUGG- -5' |
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13552 | 5' | -59 | NC_003444.1 | + | 9955 | 0.68 | 0.292527 |
Target: 5'- aCGCCgCCACCgGGCUGaaaGUCGCCu -3' miRNA: 3'- aGCGGaGGUGGgUCGGCcacUAGUGG- -5' |
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13552 | 5' | -59 | NC_003444.1 | + | 8199 | 0.68 | 0.292527 |
Target: 5'- cCGCCauugcagCCACCgC-GCCGGUGAgaACCg -3' miRNA: 3'- aGCGGa------GGUGG-GuCGGCCACUagUGG- -5' |
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13552 | 5' | -59 | NC_003444.1 | + | 13247 | 1.12 | 0.000155 |
Target: 5'- gUCGCCUCCACCCAGCCGGUGAUCACCa -3' miRNA: 3'- -AGCGGAGGUGGGUCGGCCACUAGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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