Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13553 | 5' | -51.3 | NC_003444.1 | + | 13609 | 0.66 | 0.840357 |
Target: 5'- cAGGugGCGGggcaGCgugCCGGAAACGACGa -3' miRNA: 3'- -UCU--UGCCag--CGguaGGCCUUUGUUGU- -5' |
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13553 | 5' | -51.3 | NC_003444.1 | + | 29504 | 0.66 | 0.811543 |
Target: 5'- uGAGCGGU-GCUua-CGGAAaaGCAGCAa -3' miRNA: 3'- uCUUGCCAgCGGuagGCCUU--UGUUGU- -5' |
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13553 | 5' | -51.3 | NC_003444.1 | + | 31600 | 0.67 | 0.791264 |
Target: 5'- cGAACGauacaGCCGUaCCGGGAuuaACAACAu -3' miRNA: 3'- uCUUGCcag--CGGUA-GGCCUU---UGUUGU- -5' |
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13553 | 5' | -51.3 | NC_003444.1 | + | 15491 | 0.67 | 0.78084 |
Target: 5'- uGAAUGGUCuGCCGucuUCCGGcguGGCAgauACAc -3' miRNA: 3'- uCUUGCCAG-CGGU---AGGCCu--UUGU---UGU- -5' |
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13553 | 5' | -51.3 | NC_003444.1 | + | 4738 | 0.67 | 0.748591 |
Target: 5'- -uGACGGUCGCaccaUGGAGugGGCAa -3' miRNA: 3'- ucUUGCCAGCGguagGCCUUugUUGU- -5' |
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13553 | 5' | -51.3 | NC_003444.1 | + | 30817 | 0.67 | 0.748591 |
Target: 5'- cAGAucGCGGauccUGUUAUCCGGAggaAACAACAc -3' miRNA: 3'- -UCU--UGCCa---GCGGUAGGCCU---UUGUUGU- -5' |
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13553 | 5' | -51.3 | NC_003444.1 | + | 24733 | 0.73 | 0.414377 |
Target: 5'- aAGGACGGUa-CCAgCCGGAAGCAAgGg -3' miRNA: 3'- -UCUUGCCAgcGGUaGGCCUUUGUUgU- -5' |
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13553 | 5' | -51.3 | NC_003444.1 | + | 11730 | 0.8 | 0.167023 |
Target: 5'- gGGAACaGGUCGCCGUgUGGAAACGcACGa -3' miRNA: 3'- -UCUUG-CCAGCGGUAgGCCUUUGU-UGU- -5' |
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13553 | 5' | -51.3 | NC_003444.1 | + | 14315 | 1.09 | 0.001596 |
Target: 5'- gAGAACGGUCGCCAUCCGGAAACAACAc -3' miRNA: 3'- -UCUUGCCAGCGGUAGGCCUUUGUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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