Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13554 | 3' | -54.5 | NC_003444.1 | + | 32522 | 0.66 | 0.664117 |
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Target: 5'- -aCGCGCUGggUuaaGACACagCCGuGUGc -3' miRNA: 3'- uaGCGCGACuuA---UUGUGagGGC-CGCc -5' |
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| 13554 | 3' | -54.5 | NC_003444.1 | + | 11370 | 0.66 | 0.652748 |
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Target: 5'- --gGCGCggGGAUAcACACcuuaUCCGGCGGu -3' miRNA: 3'- uagCGCGa-CUUAU-UGUGa---GGGCCGCC- -5' |
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| 13554 | 3' | -54.5 | NC_003444.1 | + | 8184 | 0.66 | 0.641359 |
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Target: 5'- -cCGCGCcggUGAGaaccggAGCGCccgUUCCGGCGGu -3' miRNA: 3'- uaGCGCG---ACUUa-----UUGUG---AGGGCCGCC- -5' |
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| 13554 | 3' | -54.5 | NC_003444.1 | + | 6316 | 0.66 | 0.623123 |
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Target: 5'- -cCGCGgaGAu--GCGCUuuaaccgcgcccgugUCCGGCGGg -3' miRNA: 3'- uaGCGCgaCUuauUGUGA---------------GGGCCGCC- -5' |
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| 13554 | 3' | -54.5 | NC_003444.1 | + | 21088 | 0.67 | 0.561977 |
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Target: 5'- cGUUGCGCUGAAUGAUACUgCaugucGCGu -3' miRNA: 3'- -UAGCGCGACUUAUUGUGAgGgc---CGCc -5' |
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| 13554 | 3' | -54.5 | NC_003444.1 | + | 3694 | 0.69 | 0.474942 |
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Target: 5'- -gCGUGCUGAGaGGCAUgacgauaaCCCGGCGc -3' miRNA: 3'- uaGCGCGACUUaUUGUGa-------GGGCCGCc -5' |
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| 13554 | 3' | -54.5 | NC_003444.1 | + | 6877 | 0.74 | 0.237044 |
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Target: 5'- gGUCGgaaaGCUGAG----ACUCCCGGCGGc -3' miRNA: 3'- -UAGCg---CGACUUauugUGAGGGCCGCC- -5' |
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| 13554 | 3' | -54.5 | NC_003444.1 | + | 11876 | 0.78 | 0.127887 |
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Target: 5'- -aCGCGCUGAAUAAaccCGgUCCCGGCa- -3' miRNA: 3'- uaGCGCGACUUAUU---GUgAGGGCCGcc -5' |
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| 13554 | 3' | -54.5 | NC_003444.1 | + | 15394 | 1.08 | 0.000823 |
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Target: 5'- cAUCGCGCUGAAUAACACUCCCGGCGGu -3' miRNA: 3'- -UAGCGCGACUUAUUGUGAGGGCCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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