Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13554 | 5' | -56.7 | NC_003444.1 | + | 26908 | 0.66 | 0.542218 |
Target: 5'- aGCCGcgCGCUGuaaaaucauuGC-CCaUCCCAGCGg -3' miRNA: 3'- cCGGUa-GCGAC----------UGuGGaAGGGUCGCg -5' |
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13554 | 5' | -56.7 | NC_003444.1 | + | 32954 | 0.66 | 0.542218 |
Target: 5'- uGCUuUCGaCUG-CACCaUCCCcuGCGCa -3' miRNA: 3'- cCGGuAGC-GACuGUGGaAGGGu-CGCG- -5' |
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13554 | 5' | -56.7 | NC_003444.1 | + | 28426 | 0.66 | 0.530278 |
Target: 5'- gGGCCAgcacucacccugaUCGCUGGCAUUaUCagCCAGCu- -3' miRNA: 3'- -CCGGU-------------AGCGACUGUGGaAG--GGUCGcg -5' |
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13554 | 5' | -56.7 | NC_003444.1 | + | 32282 | 0.66 | 0.519507 |
Target: 5'- uGGCUGauucguaaugaccUCGCgGAUGCCUUaCCUgaaAGCGCa -3' miRNA: 3'- -CCGGU-------------AGCGaCUGUGGAA-GGG---UCGCG- -5' |
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13554 | 5' | -56.7 | NC_003444.1 | + | 16884 | 0.66 | 0.509888 |
Target: 5'- uGGUCGU-GCUG-CGCCUUCuCCAGacugGCc -3' miRNA: 3'- -CCGGUAgCGACuGUGGAAG-GGUCg---CG- -5' |
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13554 | 5' | -56.7 | NC_003444.1 | + | 16146 | 0.66 | 0.503517 |
Target: 5'- cGGCgAUUGCcggugcGGCACCUUCauuaacccgcguucaUCAGCGUg -3' miRNA: 3'- -CCGgUAGCGa-----CUGUGGAAG---------------GGUCGCG- -5' |
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13554 | 5' | -56.7 | NC_003444.1 | + | 13973 | 0.67 | 0.476327 |
Target: 5'- cGGCCcgC-CUGAUgcuuaucuGCCUgaacccgaagccCCCGGCGCg -3' miRNA: 3'- -CCGGuaGcGACUG--------UGGAa-----------GGGUCGCG- -5' |
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13554 | 5' | -56.7 | NC_003444.1 | + | 4260 | 0.67 | 0.457937 |
Target: 5'- cGCCAUaCGUUGAUuaaugGCUUUCUgUAGCGCg -3' miRNA: 3'- cCGGUA-GCGACUG-----UGGAAGG-GUCGCG- -5' |
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13554 | 5' | -56.7 | NC_003444.1 | + | 8559 | 0.67 | 0.457937 |
Target: 5'- cGGUCAUCGUga--ACCguuUCCCcuucGGCGCa -3' miRNA: 3'- -CCGGUAGCGacugUGGa--AGGG----UCGCG- -5' |
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13554 | 5' | -56.7 | NC_003444.1 | + | 12435 | 0.67 | 0.437958 |
Target: 5'- uGCCGagGCUGACACCggaa-AGCGUu -3' miRNA: 3'- cCGGUagCGACUGUGGaagggUCGCG- -5' |
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13554 | 5' | -56.7 | NC_003444.1 | + | 28956 | 0.67 | 0.437958 |
Target: 5'- -cCCAUCGCaGAUACCcagCCC-GCGUa -3' miRNA: 3'- ccGGUAGCGaCUGUGGaa-GGGuCGCG- -5' |
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13554 | 5' | -56.7 | NC_003444.1 | + | 30545 | 0.68 | 0.39398 |
Target: 5'- gGGCCugaaaguaugaaGCUGAUccuGCCUUucccgCCCAGCGUg -3' miRNA: 3'- -CCGGuag---------CGACUG---UGGAA-----GGGUCGCG- -5' |
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13554 | 5' | -56.7 | NC_003444.1 | + | 15155 | 0.68 | 0.389374 |
Target: 5'- cGCCggagcguGUCGCUGACGuCCagCCUGGCGa -3' miRNA: 3'- cCGG-------UAGCGACUGU-GGaaGGGUCGCg -5' |
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13554 | 5' | -56.7 | NC_003444.1 | + | 15398 | 0.68 | 0.381174 |
Target: 5'- aGGUCAUCGCgcUGAauaACacUCCCGGCGg -3' miRNA: 3'- -CCGGUAGCG--ACUg--UGgaAGGGUCGCg -5' |
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13554 | 5' | -56.7 | NC_003444.1 | + | 16287 | 0.7 | 0.329521 |
Target: 5'- cGGCgGgaaCGCaGAC-CCUUCgCCAGCGUc -3' miRNA: 3'- -CCGgUa--GCGaCUGuGGAAG-GGUCGCG- -5' |
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13554 | 5' | -56.7 | NC_003444.1 | + | 30459 | 0.7 | 0.31349 |
Target: 5'- gGGCCAcgccacgcuuuaUCGUgaUGAUGCCUUuuuucUCCAGUGCg -3' miRNA: 3'- -CCGGU------------AGCG--ACUGUGGAA-----GGGUCGCG- -5' |
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13554 | 5' | -56.7 | NC_003444.1 | + | 16362 | 0.7 | 0.293543 |
Target: 5'- aGCCGUUGCUGACGCUggcgaagggucugcgUUCCCGcCGg -3' miRNA: 3'- cCGGUAGCGACUGUGG---------------AAGGGUcGCg -5' |
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13554 | 5' | -56.7 | NC_003444.1 | + | 15359 | 1.14 | 0.000174 |
Target: 5'- aGGCCAUCGCUGACACCUUCCCAGCGCa -3' miRNA: 3'- -CCGGUAGCGACUGUGGAAGGGUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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