Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13555 | 3' | -54.7 | NC_003444.1 | + | 382 | 0.66 | 0.670025 |
Target: 5'- aCGCCGccAGAacucGUAGGCCAcgCACCa -3' miRNA: 3'- -GCGGCcuUCUgc--CGUCUGGUaaGUGG- -5' |
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13555 | 3' | -54.7 | NC_003444.1 | + | 16054 | 0.66 | 0.63626 |
Target: 5'- cCGCCGGGGcaagcaacuGAUGGCuGuauuccCCGUUCGCa -3' miRNA: 3'- -GCGGCCUU---------CUGCCGuCu-----GGUAAGUGg -5' |
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13555 | 3' | -54.7 | NC_003444.1 | + | 16472 | 0.66 | 0.63626 |
Target: 5'- gCGCCGGAAuGGCgguauuacuGGCAGgucaacauGCCAgcugcCACCa -3' miRNA: 3'- -GCGGCCUU-CUG---------CCGUC--------UGGUaa---GUGG- -5' |
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13555 | 3' | -54.7 | NC_003444.1 | + | 14834 | 0.66 | 0.635132 |
Target: 5'- aGCCGGGAGccguaACGGUcauucuguaccgcAGGCCAgguaucccccCACCa -3' miRNA: 3'- gCGGCCUUC-----UGCCG-------------UCUGGUaa--------GUGG- -5' |
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13555 | 3' | -54.7 | NC_003444.1 | + | 36705 | 0.67 | 0.613712 |
Target: 5'- aGCCGcGAAcGCGGCAGGCUuuuggaGUUCGa- -3' miRNA: 3'- gCGGC-CUUcUGCCGUCUGG------UAAGUgg -5' |
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13555 | 3' | -54.7 | NC_003444.1 | + | 9311 | 0.67 | 0.602458 |
Target: 5'- aGCCaGGcuGACGaCAauacauagaccuGACCGUUCGCCa -3' miRNA: 3'- gCGG-CCuuCUGCcGU------------CUGGUAAGUGG- -5' |
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13555 | 3' | -54.7 | NC_003444.1 | + | 8182 | 0.67 | 0.580039 |
Target: 5'- gCGCCGGugagaaccGGAgcgcccguucCGGCGGucCCcgUCGCCa -3' miRNA: 3'- -GCGGCCu-------UCU----------GCCGUCu-GGuaAGUGG- -5' |
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13555 | 3' | -54.7 | NC_003444.1 | + | 17536 | 0.68 | 0.556696 |
Target: 5'- uGuuGGAgaaGGACGGuCAacaaugacaggauGACCGUucUCACCa -3' miRNA: 3'- gCggCCU---UCUGCC-GU-------------CUGGUA--AGUGG- -5' |
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13555 | 3' | -54.7 | NC_003444.1 | + | 70 | 0.68 | 0.546773 |
Target: 5'- gGCUGGA--ACGGCGGGgCGUuccgggcgUCGCCc -3' miRNA: 3'- gCGGCCUucUGCCGUCUgGUA--------AGUGG- -5' |
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13555 | 3' | -54.7 | NC_003444.1 | + | 7667 | 0.68 | 0.535814 |
Target: 5'- uGCCGu----CGGCAGACUAUgcaCGCCa -3' miRNA: 3'- gCGGCcuucuGCCGUCUGGUAa--GUGG- -5' |
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13555 | 3' | -54.7 | NC_003444.1 | + | 13442 | 0.68 | 0.514136 |
Target: 5'- aCGUCaGGAAuaacacccGACGGCGcACCGUuguUCACCa -3' miRNA: 3'- -GCGG-CCUU--------CUGCCGUcUGGUA---AGUGG- -5' |
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13555 | 3' | -54.7 | NC_003444.1 | + | 16187 | 0.69 | 0.46165 |
Target: 5'- uCGCCGGAucGCuGGCuGACCAUgcaggACCg -3' miRNA: 3'- -GCGGCCUucUG-CCGuCUGGUAag---UGG- -5' |
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13555 | 3' | -54.7 | NC_003444.1 | + | 13251 | 0.7 | 0.421769 |
Target: 5'- uGCCGGAcguagucuGACGGCuGACCugAUUgACUg -3' miRNA: 3'- gCGGCCUu-------CUGCCGuCUGG--UAAgUGG- -5' |
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13555 | 3' | -54.7 | NC_003444.1 | + | 16328 | 0.7 | 0.412127 |
Target: 5'- cCGCCGGAAuacauucaucCGGCAGACCg--CACa -3' miRNA: 3'- -GCGGCCUUcu--------GCCGUCUGGuaaGUGg -5' |
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13555 | 3' | -54.7 | NC_003444.1 | + | 12668 | 0.71 | 0.374957 |
Target: 5'- uGCCGGAugcguGACGGaAGGCCA---GCCa -3' miRNA: 3'- gCGGCCUu----CUGCCgUCUGGUaagUGG- -5' |
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13555 | 3' | -54.7 | NC_003444.1 | + | 1109 | 0.72 | 0.331789 |
Target: 5'- cCGuCCGGAGGAUGGCGcGCgGUUUgaACCg -3' miRNA: 3'- -GC-GGCCUUCUGCCGUcUGgUAAG--UGG- -5' |
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13555 | 3' | -54.7 | NC_003444.1 | + | 16269 | 0.72 | 0.313986 |
Target: 5'- uGCCGGAugaauguauuccGGCGggaacGCAGACCcUUCGCCa -3' miRNA: 3'- gCGGCCUu-----------CUGC-----CGUCUGGuAAGUGG- -5' |
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13555 | 3' | -54.7 | NC_003444.1 | + | 29981 | 0.73 | 0.292387 |
Target: 5'- gGCCGGAAGAU-GCAGAUCAUaUugCu -3' miRNA: 3'- gCGGCCUUCUGcCGUCUGGUAaGugG- -5' |
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13555 | 3' | -54.7 | NC_003444.1 | + | 15551 | 1.13 | 0.000371 |
Target: 5'- aCGCCGGAAGACGGCAGACCAUUCACCg -3' miRNA: 3'- -GCGGCCUUCUGCCGUCUGGUAAGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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