Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13555 | 5' | -47.7 | NC_003444.1 | + | 31730 | 0.66 | 0.969056 |
Target: 5'- cGGGAcUGGUGAUGUucuuGCCGaacaugcagccuuUCGccgUCAGCg -3' miRNA: 3'- -UCCUaAUUACUGCA----UGGC-------------AGC---AGUCG- -5' |
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13555 | 5' | -47.7 | NC_003444.1 | + | 7368 | 0.66 | 0.969056 |
Target: 5'- aAGGGUUAuuucgguGUGAUGUucgauAUCGcCGUCAGg -3' miRNA: 3'- -UCCUAAU-------UACUGCA-----UGGCaGCAGUCg -5' |
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13555 | 5' | -47.7 | NC_003444.1 | + | 9247 | 0.66 | 0.965826 |
Target: 5'- cAGGucu----AUGUAUUGUCGUCAGCc -3' miRNA: 3'- -UCCuaauuacUGCAUGGCAGCAGUCG- -5' |
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13555 | 5' | -47.7 | NC_003444.1 | + | 16215 | 0.67 | 0.932442 |
Target: 5'- cGGGUUAAUGAagGUGCCG-CacCGGCa -3' miRNA: 3'- uCCUAAUUACUg-CAUGGCaGcaGUCG- -5' |
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13555 | 5' | -47.7 | NC_003444.1 | + | 10979 | 0.71 | 0.768072 |
Target: 5'- uGGGAUUGAUgGGCGUugGCgGUgccaucaguauUGUCAGCa -3' miRNA: 3'- -UCCUAAUUA-CUGCA--UGgCA-----------GCAGUCG- -5' |
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13555 | 5' | -47.7 | NC_003444.1 | + | 15516 | 1.14 | 0.001918 |
Target: 5'- cAGGAUUAAUGACGUACCGUCGUCAGCg -3' miRNA: 3'- -UCCUAAUUACUGCAUGGCAGCAGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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