miRNA: 3'- -GGaCgUUGUACAGUuu--CAUCAGUAgu -5'
miRNA: 3'- ggACGUUGU------ACAGUUUCAU---CAGUAGu -5'
miRNA: 3'- gGACGUUGUACaGUUUCau----CAGUagu -5'
miRNA: 3'- ggaCGUUguaCAGUuuCAUCAGUAGU- -5'
miRNA: 3'- -GGACGUUGUACAGUUUCAUCAGUAGU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13559 | 5' | -44.8 | NC_003444.1 | + | 32152 | 0.66 | 0.99305 |
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Target: 5'- gCCcGgAGCGUGUCGcauuGUGGUCAUg- -3' miRNA: 3'- -GGaCgUUGUACAGUuu--CAUCAGUAgu -5' |
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| 13559 | 5' | -44.8 | NC_003444.1 | + | 8702 | 0.66 | 0.990426 |
|
Target: 5'- -aUGCAGCGucucgcUGUCGAGGUAgcuGUUAUCc -3' miRNA: 3'- ggACGUUGU------ACAGUUUCAU---CAGUAGu -5' |
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| 13559 | 5' | -44.8 | NC_003444.1 | + | 36803 | 0.68 | 0.968066 |
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Target: 5'- gCUGCAACGUGaCAAAGgccuguGUCAg-- -3' miRNA: 3'- gGACGUUGUACaGUUUCau----CAGUagu -5' |
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| 13559 | 5' | -44.8 | NC_003444.1 | + | 6614 | 0.7 | 0.945821 |
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Target: 5'- --aGCAAaacGUCGucGUAGUCAUCAu -3' miRNA: 3'- ggaCGUUguaCAGUuuCAUCAGUAGU- -5' |
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| 13559 | 5' | -44.8 | NC_003444.1 | + | 16824 | 1.12 | 0.00484 |
|
Target: 5'- uCCUGCAACAUGUCAAAGUAGUCAUCAu -3' miRNA: 3'- -GGACGUUGUACAGUUUCAUCAGUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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