Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13563 | 3' | -53.6 | NC_003444.1 | + | 4051 | 0.66 | 0.641412 |
Target: 5'- aGCGAaagAUGaCUGGUuaauaucuccacguuGCaucgGGGCCAGCAc -3' miRNA: 3'- -CGCUa--UAC-GACUAu--------------CGa---CCCGGUCGU- -5' |
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13563 | 3' | -53.6 | NC_003444.1 | + | 828 | 0.68 | 0.525194 |
Target: 5'- uGUGAUcUGCUGugcguuUGGCUGGGUCAa-- -3' miRNA: 3'- -CGCUAuACGACu-----AUCGACCCGGUcgu -5' |
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13563 | 3' | -53.6 | NC_003444.1 | + | 13174 | 0.69 | 0.48012 |
Target: 5'- gGUGAUGaGCUGGUgaucaccGGCUGGGUggaGGCGa -3' miRNA: 3'- -CGCUAUaCGACUA-------UCGACCCGg--UCGU- -5' |
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13563 | 3' | -53.6 | NC_003444.1 | + | 28444 | 1.11 | 0.000528 |
Target: 5'- aGCGAUAUGCUGAUAGCUGGGCCAGCAc -3' miRNA: 3'- -CGCUAUACGACUAUCGACCCGGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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