Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13565 | 3' | -59.7 | NC_003444.1 | + | 15272 | 0.69 | 0.236008 |
Target: 5'- gGGC-CAcGGCUgCCAGCGGGuAUAUGCgCu -3' miRNA: 3'- -CCGaGU-CCGA-GGUCGUCC-UGUGCGgG- -5' |
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13565 | 3' | -59.7 | NC_003444.1 | + | 30185 | 0.67 | 0.303759 |
Target: 5'- uGGCgCAGGUucgCCAGCAGGcggaaaaACugGUCa -3' miRNA: 3'- -CCGaGUCCGa--GGUCGUCC-------UGugCGGg -5' |
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13565 | 3' | -59.7 | NC_003444.1 | + | 31934 | 1.12 | 0.000126 |
Target: 5'- uGGCUCAGGCUCCAGCAGGACACGCCCc -3' miRNA: 3'- -CCGAGUCCGAGGUCGUCCUGUGCGGG- -5' |
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13565 | 3' | -59.7 | NC_003444.1 | + | 34399 | 0.66 | 0.360967 |
Target: 5'- aGGUaaAGGCuUCCGGCAGGuuucaACAccCGCCa -3' miRNA: 3'- -CCGagUCCG-AGGUCGUCC-----UGU--GCGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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