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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13567 | 3' | -54.9 | NC_003444.1 | + | 21193 | 0.66 | 0.624593 |
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Target: 5'- gGCUCCGGAcaUUCAucaaucagcCUUUguGCuucauggggcuccaGCCAGCg -3' miRNA: 3'- -UGAGGCCU--AGGU---------GAAAguCG--------------CGGUCG- -5' |
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| 13567 | 3' | -54.9 | NC_003444.1 | + | 12836 | 0.66 | 0.593861 |
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Target: 5'- -aUCCGcauucaGAUCUGCUUUCacccggcgcAGCGCCAGa -3' miRNA: 3'- ugAGGC------CUAGGUGAAAG---------UCGCGGUCg -5' |
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| 13567 | 3' | -54.9 | NC_003444.1 | + | 32107 | 0.66 | 0.582532 |
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Target: 5'- cGCUCCGGGcgcaaUCCG---UCuGUGCUGGCa -3' miRNA: 3'- -UGAGGCCU-----AGGUgaaAGuCGCGGUCG- -5' |
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| 13567 | 3' | -54.9 | NC_003444.1 | + | 36491 | 0.68 | 0.494212 |
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Target: 5'- -gUCUGGAUCUgaaugugagccuGCUUUCGGCGUUGGg -3' miRNA: 3'- ugAGGCCUAGG------------UGAAAGUCGCGGUCg -5' |
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| 13567 | 3' | -54.9 | NC_003444.1 | + | 14495 | 0.68 | 0.47305 |
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Target: 5'- gACUCCGGcggcaCCACcaugUCAGCGaugCGGCu -3' miRNA: 3'- -UGAGGCCua---GGUGaa--AGUCGCg--GUCG- -5' |
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| 13567 | 3' | -54.9 | NC_003444.1 | + | 24040 | 0.68 | 0.47305 |
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Target: 5'- uUUCCGuGGUgCGCUUUCcacUGCCAGCa -3' miRNA: 3'- uGAGGC-CUAgGUGAAAGuc-GCGGUCG- -5' |
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| 13567 | 3' | -54.9 | NC_003444.1 | + | 7137 | 0.68 | 0.47305 |
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Target: 5'- cGCUCCGGGUUC-----CAGC-CCAGCa -3' miRNA: 3'- -UGAGGCCUAGGugaaaGUCGcGGUCG- -5' |
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| 13567 | 3' | -54.9 | NC_003444.1 | + | 32502 | 1.12 | 0.000353 |
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Target: 5'- gACUCCGGAUCCACUUUCAGCGCCAGCa -3' miRNA: 3'- -UGAGGCCUAGGUGAAAGUCGCGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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