Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13568 | 5' | -54.9 | NC_003444.1 | + | 8166 | 0.67 | 0.525902 |
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Target: 5'- gAGCGCCCGuuCCGGCgGUCCccg-UCGCc -3' miRNA: 3'- -UUGUGGGU--GGUCGgCGGGuaaaAGUG- -5' |
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| 13568 | 5' | -54.9 | NC_003444.1 | + | 24654 | 0.67 | 0.482068 |
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Target: 5'- gAACACCUGCgguggCAGCCGCCCGg------ -3' miRNA: 3'- -UUGUGGGUG-----GUCGGCGGGUaaaagug -5' |
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| 13568 | 5' | -54.9 | NC_003444.1 | + | 20105 | 0.69 | 0.400251 |
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Target: 5'- gAGCAUUUAUCAGCUGCCCAaccaacaaUCACa -3' miRNA: 3'- -UUGUGGGUGGUCGGCGGGUaaa-----AGUG- -5' |
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| 13568 | 5' | -54.9 | NC_003444.1 | + | 13841 | 0.69 | 0.400251 |
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Target: 5'- -uCAUCCGuCCGcGCCGCCCGUUgUCGg -3' miRNA: 3'- uuGUGGGU-GGU-CGGCGGGUAAaAGUg -5' |
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| 13568 | 5' | -54.9 | NC_003444.1 | + | 25602 | 0.71 | 0.311332 |
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Target: 5'- gGACGCCCACgaagUAGCCGCCUGggg-CAUa -3' miRNA: 3'- -UUGUGGGUG----GUCGGCGGGUaaaaGUG- -5' |
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| 13568 | 5' | -54.9 | NC_003444.1 | + | 34021 | 0.73 | 0.207405 |
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Target: 5'- uAGCACCUGCCAGCCauucGCUCAUgcuuacuggUCACa -3' miRNA: 3'- -UUGUGGGUGGUCGG----CGGGUAaa-------AGUG- -5' |
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| 13568 | 5' | -54.9 | NC_003444.1 | + | 34056 | 1.08 | 0.000582 |
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Target: 5'- cAACACCCACCAGCCGCCCAUUUUCACa -3' miRNA: 3'- -UUGUGGGUGGUCGGCGGGUAAAAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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