Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13570 | 5' | -56.8 | NC_003444.1 | + | 6643 | 0.67 | 0.403296 |
Target: 5'- -----gUGCAuCCGGU-GGCAGCGGCu -3' miRNA: 3'- augaagGCGUuGGCCAgUCGUCGCCG- -5' |
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13570 | 5' | -56.8 | NC_003444.1 | + | 29848 | 0.66 | 0.462599 |
Target: 5'- aUACagCCGC-GCCaGUCAGCaugAGgGGCa -3' miRNA: 3'- -AUGaaGGCGuUGGcCAGUCG---UCgCCG- -5' |
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13570 | 5' | -56.8 | NC_003444.1 | + | 36425 | 1.1 | 0.000284 |
Target: 5'- gUACUUCCGCAACCGGUCAGCAGCGGCa -3' miRNA: 3'- -AUGAAGGCGUUGGCCAGUCGUCGCCG- -5' |
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13570 | 5' | -56.8 | NC_003444.1 | + | 13718 | 0.68 | 0.375504 |
Target: 5'- gUGCgggcCCGCAGCgCGGU-GGUGGUGGCc -3' miRNA: 3'- -AUGaa--GGCGUUG-GCCAgUCGUCGCCG- -5' |
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13570 | 5' | -56.8 | NC_003444.1 | + | 6897 | 0.66 | 0.504572 |
Target: 5'- aACUgugCCGCGccugccacCCGGUUuucaaaauacgGGCAGCGGg -3' miRNA: 3'- aUGAa--GGCGUu-------GGCCAG-----------UCGUCGCCg -5' |
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13570 | 5' | -56.8 | NC_003444.1 | + | 14284 | 0.66 | 0.515325 |
Target: 5'- cGCUUCCGC-ACCGcuGUUuGCccuuGCGGUa -3' miRNA: 3'- aUGAAGGCGuUGGC--CAGuCGu---CGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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