Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13572 | 3' | -52.9 | NC_003444.1 | + | 6467 | 0.67 | 0.705456 |
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Target: 5'- ----aCCCGGUGccgaccgcgcAUGGcgCAUCACCa -3' miRNA: 3'- cguugGGGCCAC----------UACCaaGUAGUGGg -5' |
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| 13572 | 3' | -52.9 | NC_003444.1 | + | 15392 | 0.67 | 0.694248 |
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Target: 5'- uCAGCUCCGGUGGUGuccugcgcGUgccgAUCGCCUg -3' miRNA: 3'- cGUUGGGGCCACUAC--------CAag--UAGUGGG- -5' |
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| 13572 | 3' | -52.9 | NC_003444.1 | + | 28450 | 0.67 | 0.694248 |
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Target: 5'- uGCGGCCCuCuGUGAguccgggcUGGUaaaaaaaGUCACCCg -3' miRNA: 3'- -CGUUGGG-GcCACU--------ACCAag-----UAGUGGG- -5' |
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| 13572 | 3' | -52.9 | NC_003444.1 | + | 6310 | 0.68 | 0.614658 |
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Target: 5'- aGCAGCCCgCGGaGAUGcGcUUUAaccgCGCCCg -3' miRNA: 3'- -CGUUGGG-GCCaCUAC-C-AAGUa---GUGGG- -5' |
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| 13572 | 3' | -52.9 | NC_003444.1 | + | 32633 | 0.68 | 0.603265 |
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Target: 5'- cGUGGCCUaucaGGUGGUGGggaUCAUCAgCa -3' miRNA: 3'- -CGUUGGGg---CCACUACCa--AGUAGUgGg -5' |
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| 13572 | 3' | -52.9 | NC_003444.1 | + | 13709 | 0.69 | 0.591899 |
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Target: 5'- cGCAGCg-CGGUGGUGGUggCcUCACCg -3' miRNA: 3'- -CGUUGggGCCACUACCAa-GuAGUGGg -5' |
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| 13572 | 3' | -52.9 | NC_003444.1 | + | 434 | 0.69 | 0.558057 |
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Target: 5'- cGCccauuGCaCCGGUGAUGGU--GUUGCCCu -3' miRNA: 3'- -CGu----UGgGGCCACUACCAagUAGUGGG- -5' |
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| 13572 | 3' | -52.9 | NC_003444.1 | + | 15941 | 0.71 | 0.460764 |
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Target: 5'- cGCAACuCCgCGGUGAUGGca----GCCCg -3' miRNA: 3'- -CGUUG-GG-GCCACUACCaaguagUGGG- -5' |
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| 13572 | 3' | -52.9 | NC_003444.1 | + | 1146 | 1.13 | 0.000591 |
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Target: 5'- gGCAACCCCGGUGAUGGUUCAUCACCCc -3' miRNA: 3'- -CGUUGGGGCCACUACCAAGUAGUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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