Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13573 | 5' | -49.4 | NC_003444.1 | + | 31870 | 0.66 | 0.868833 |
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Target: 5'- aGGGGCGuguCC-UGCUGgaGCCugAGCCa -3' miRNA: 3'- -UUUUGUuuuGGuACGACgaCGG--UCGG- -5' |
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| 13573 | 5' | -49.4 | NC_003444.1 | + | 3943 | 0.66 | 0.850679 |
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Target: 5'- gGAGACAcAGACCAUucgugauCUGgUGCUGGCCc -3' miRNA: 3'- -UUUUGU-UUUGGUAc------GACgACGGUCGG- -5' |
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| 13573 | 5' | -49.4 | NC_003444.1 | + | 32533 | 0.66 | 0.84118 |
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Target: 5'- uAAGACAcAGCCGUGUgcaUGUUGUggaaAGCCu -3' miRNA: 3'- -UUUUGUuUUGGUACG---ACGACGg---UCGG- -5' |
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| 13573 | 5' | -49.4 | NC_003444.1 | + | 19658 | 0.67 | 0.821393 |
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Target: 5'- -uAGCAAGcACCAUGUcagcacCUGCCuGCCa -3' miRNA: 3'- uuUUGUUU-UGGUACGac----GACGGuCGG- -5' |
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| 13573 | 5' | -49.4 | NC_003444.1 | + | 36643 | 0.69 | 0.710259 |
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Target: 5'- -----cGGACUAucUGaCUGCUGCCAGCa -3' miRNA: 3'- uuuuguUUUGGU--AC-GACGACGGUCGg -5' |
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| 13573 | 5' | -49.4 | NC_003444.1 | + | 5099 | 0.7 | 0.655082 |
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Target: 5'- -cGGCAGu-CUGUGUugugccggucacugaUGCUGCCAGCCc -3' miRNA: 3'- uuUUGUUuuGGUACG---------------ACGACGGUCGG- -5' |
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| 13573 | 5' | -49.4 | NC_003444.1 | + | 22050 | 0.71 | 0.601764 |
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Target: 5'- aAAAugGAuACCAgcucGUUGCUGCCAGaCg -3' miRNA: 3'- -UUUugUUuUGGUa---CGACGACGGUCgG- -5' |
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| 13573 | 5' | -49.4 | NC_003444.1 | + | 9591 | 0.72 | 0.495563 |
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Target: 5'- -cGACAGAGCUuUGCgGCacuuucGCCAGCCg -3' miRNA: 3'- uuUUGUUUUGGuACGaCGa-----CGGUCGG- -5' |
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| 13573 | 5' | -49.4 | NC_003444.1 | + | 3151 | 1.12 | 0.001141 |
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Target: 5'- gAAAACAAAACCAUGCUGCUGCCAGCCg -3' miRNA: 3'- -UUUUGUUUUGGUACGACGACGGUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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